The Arabidopsis alkaline ceramidase TOD1 is a key turgor pressure regulator in plant cells

被引：54

作者：

Chen, Li-Yu ^{[1
,2
,3
]}

Shi, Dong-Qiao ^{[1
,2
]}

Zhang, Wen-Juan ^{[1
,2
,3
]}

Tang, Zuo-Shun ^{[1
,2
]}

Liu, Jie ^{[1
,2
]}

Yang, Wei-Cai ^{[1
,2
,4
]}

机构：

[1] Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100101, Peoples R China

[2] Chinese Acad Sci, Natl Ctr Plant Gene Res Beijing, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[4] Fudan Univ, Collaborat Innovat Ctr Genet & Dev, Shanghai 200433, Peoples R China

来源：

NATURE COMMUNICATIONS | 2015年 / 6卷

基金：

中国国家自然科学基金;

关键词：

POLLEN-TUBE GROWTH; FUNCTIONAL-CHARACTERIZATION; SACCHAROMYCES-CEREVISIAE; ENDOPLASMIC-RETICULUM; PROTEIN FAMILIES; ORGANELLE PH; CLONING; ENZYME; GENE; SPHINGOSINE;

D O I：

10.1038/ncomms7030

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Turgor pressure plays pivotal roles in the growth and movement of walled cells that make up plants and fungi. However, the molecular mechanisms regulating turgor pressure and the coordination between turgor pressure and cell wall remodelling for cell growth remain poorly understood. Here, we report the characterization of Arabidopsis TurgOr regulation Defect 1 (TOD1), which is preferentially expressed in pollen tubes and silique guard cells. We demonstrate that TOD1 is a Golgi-localized alkaline ceramidase. tod1 mutant pollen tubes have higher turgor than wild type and show growth retardation both in pistils and in agarose medium. In addition, tod1 guard cells are insensitive to abscisic acid (ABA)-induced stomatal closure, whereas sphingosine-1-phosphate, a putative downstream component of ABA signalling and product of alkaline ceramidases, promotes closure in both wild type and tod1. Our data suggest that TOD1 acts in turgor pressure regulation in both guard cells and pollen tubes.

引用

页数：10