Keeping qRT-PCR rigorous and biologically relevant

被引：26

作者：

Bennett, John

Hondred, David ^{[1
]}

机构：

[1] DuPont Pioneer, Johnston, IA 50131 USA

来源：

PLANT CELL REPORTS | 2015年 / 34卷 / 01期

关键词：

REFERENCE GENES; IDENTIFICATION; NORMALIZATION; GUIDELINES;

D O I：

10.1007/s00299-014-1692-6

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

引用

页码：1 / 3

页数：3

共 8 条

[1] MIQE precis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments [J].

Bustin, Stephen A. ;

Beaulieu, Jean-Francois ;

Huggett, Jim ;

Jaggi, Rolf ;

Kibenge, Frederick S. B. ;

Olsvik, Pal A. ;

Penning, Louis C. ;

Toegel, Stefan .

BMC MOLECULAR BIOLOGY, 2010, 11

[2] The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments [J].

Bustin, Stephen A. ;

Benes, Vladimir ;

Garson, Jeremy A. ;

Hellemans, Jan ;

Huggett, Jim ;

Kubista, Mikael ;

Mueller, Reinhold ;

Nolan, Tania ;

Pfaffl, Michael W. ;

Shipley, Gregory L. ;

Vandesompele, Jo ;

Wittwer, Carl T. .

CLINICAL CHEMISTRY, 2009, 55 (04) :611-622

[3] Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis [J].

Czechowski, T ;

Stitt, M ;

Altmann, T ;

Udvardi, MK ;

Scheible, WR .

PLANT PHYSIOLOGY, 2005, 139 (01) :5-17

[4] How to perform RT-qPCR accurately in plant species? A case study on flower colour gene expression in an azalea (Rhododendron simsii hybrids) mapping population [J].

De Keyser, Ellen ;

Desmet, Laurence ;

Van Bockstaele, Erik ;

De Riek, Jan .

BMC MOLECULAR BIOLOGY, 2013, 14

[5] Identification of potential target genes for the tomato fruit-ripening regulator RIN by chromatin immunoprecipitation [J].

Fujisawa, Masaki ;

Nakano, Toshitsugu ;

Ito, Yasuhiro .

BMC PLANT BIOLOGY, 2011, 11

[6] RefGenes: identification of reliable and condition specific reference genes for RT-qPCR data normalization [J].

Hruz, Tomas ;

Wyss, Markus ;

Docquier, Mylene ;

Pfaffl, Michael W. ;

Masanetz, Sabine ;

Borghi, Lorenzo ;

Verbrugghe, Phebe ;

Kalaydjieva, Luba ;

Bleuler, Stefan ;

Laule, Oliver ;

Descombes, Patrick ;

Gruissem, Wilhelm ;

Zimmermann, Philip .

BMC GENOMICS, 2011, 12

[7] Assessing Reference Genes for Accurate Transcript Normalization Using Quantitative Real-Time PCR in Pearl Millet [Pennisetum glaucum (L.) R. Br.] [J].

Saha, Prasenjit ;

Blumwald, Eduardo .

PLOS ONE, 2014, 9 (08)

[8] Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes [J].

Vandesompele, Jo ;

De Preter, Katleen ;

Pattyn, Filip ;

Poppe, Bruce ;

Van Roy, Nadine ;

De Paepe, Anne ;

Speleman, Frank .

GENOME BIOLOGY, 2002, 3 (07)

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