Highly sensitive/selective 3D nanostructured immunoparticle-based interface on a multichannel sensor array for detecting amyloid-beta in Alzheimer's disease

被引：26

作者：

Liu, Ta-Chung ^{[1
]}

Lee, Yi-Chao ^{[2
]}

Ko, Chiung-Yuan ^{[2
]}

Liu, Ren-Shyan ^{[3
,4
,5
]}

Ke, Chien-Chih ^{[3
,4
,5
]}

Lo, Yu-Chun ^{[2
]}

Hong, Pei-Sung ^{[1
]}

Chu, Chao-Yi ^{[1
]}

Chang, Ching-Wen ^{[6
]}

Wu, Pu-Wei ^{[1
]}

Chen, You-Yin ^{[2
,6
]}

Chen, San-Yuan ^{[1
,7
]}

机构：

[1] Natl Chiao Tung Univ, Dept Mat Sci & Engn, 1001 Ta Hsueh Rd, Hsinchu 30010, Taiwan

[2] Taipei Med Univ, PhD Program Neural Regenerat Med, 250 Wu Xing St, Taipei 11010, Taiwan

[3] Natl Yang Ming Univ, Dept Biomed Imaging & Radiol Sci, 155,Sec 2,Linong St, Taipei 11221, Taiwan

[4] Taipei Vet Gen Hosp, Dept Nucl Med, 201,Sec 2,Shipai Rd, Taipei 11217, Taiwan

[5] Taipei Vet Gen Hosp, Natl PET Cyclotron Ctr, 201,Sec 2,Shipai Rd, Taipei 11217, Taiwan

[6] Natl Yang Ming Univ, Dept Biomed Engn, 155,Sec 2,Linong St, Taipei 11221, Taiwan

[7] Natl Tsing Hua Univ, Frontier Res Ctr Fundamental & Appl Sci Matters, Hsinchu 30010, Taiwan

来源：

THERANOSTICS | 2018年 / 8卷 / 15期

关键词：

point-of-care testing (PoCT); Alzheimer's disease; amyloid-beta (A beta); silk fibroin; microPET; SILK-BASED BIOMATERIALS; BLOOD-BRAIN-BARRIER; A-BETA; ELECTROCHEMICAL DETECTION; AGGREGATION; PEPTIDE; BIOSENSOR; MICE; BIOMARKERS; A-BETA-42;

D O I：

10.7150/thno.25625

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Accumulation of beta-amyloid (A beta) peptides is highly associated with Alzheimer's disease (AD) progression in prevailing studies. The successful development of an ultrasensitive detection assay for A beta is a challenging task, especially from blood-based samples. Methods: We have developed a one-step electrophoresis/electropolymerization strategy for preparing a CSIP hierarchical immunoelectrochemical interface that is easily integrated into a PoCT device. The interface includes conductive silk fibroin-based immunoparticles (CSIPs) via electropolymerized Poly(3,4-ethylenedioxythiophene) (PEDOT) bridging to enable on-site electrochemical detection of serum amyloid-beta(42) (A beta(42)) and -beta(40) (A beta(40)) peptides from an AD blood test. In addition, micro-positron emission tomography (microPET) neuroimaging and behavioral tests were simultaneously performed. Results: This nanostructured conductive interface favors penetration of water-soluble biomolecules and catalyzes a redox reaction, providing limits of detection (LOD) of 6.63 pg/mL for A beta(40) and 3.74 pg/mL for A beta(42). Our proof-of-concept study confirms that the multi-sensing electrochemical immunosensor array (MEIA) platform enables simultaneous measurement of serum A beta(42) and A beta(40) peptide levels and is more informative in early stage AD animals than amyloid-labeling A beta plaque PET imaging and behavioral tests. Conclusion: We believe this study greatly expands the applications of silk fibroin-based materials, is an important contribution to the advancement of biomaterials, and would also be valuable in the design of new types of multichannel electrochemical immunosensor arrays for the detection of other diseases.

引用

页码：4210 / 4225

页数：16

共 42 条

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