Invivo detection of triacylglycerols through Nile red staining and quantification of fatty acids in hyper lipid producer Nannochloropsis sp. cultured under adequate nitrogen and deficient nitrogen condition

被引：11

作者：

Ahamed, Tharifkhan Shan ^{[1
]}

Brindhadevi, Kathirvel ^{[2
]}

Krishnan, Ramakrishnan ^{[3
]}

Phuong, Tran Nhat ^{[4
]}

Alharbi, Sulaiman Ali ^{[5
]}

Chinnathambi, Arunachalam ^{[5
]}

Mathimani, Thangavel ^{[6
]}

机构：

[1] Van Lang Univ, Sch Engn & Technol, Chem Engn Adv Mat & Renewable Energy Res Grp, Ho Chi Minh City, Vietnam

[2] Saveetha Univ, Saveetha Inst Med & Tech Sci, Saveetha Dent Coll, Ctr Transdisciplinary Res CFTR,Dept Pharmacol, Chennai, India

[3] Harrisburg Univ Sci & Technol, Dept Business, Harrisburg, PA 17101 USA

[4] Van Lang Univ, Fac Med, Ho Chi Minh City, Vietnam

[5] King Saud Univ, Coll Sci, Dept Bot & Microbiol, POB 2455, Riyadh 11451, Saudi Arabia

[6] Natl Inst Technol, Dept Energy & Environm, Tiruchirappalli 620015, Tamil Nadu, India

来源：

FUEL | 2022年 / 322卷

关键词：

Nannochloropsis; Nile red; TAG; Triacylglycerols; FTIR; FAME-Fatty acid methyl esters; Biodiesel; CHLORELLA-VULGARIS; BIOFUEL PRODUCTION; BIODIESEL; ACCUMULATION; MICROALGAE; ALGAE; CHLAMYDOMONAS; STARVATION; GROWTH; CULTIVATION;

D O I：

10.1016/j.fuel.2022.124179

中图分类号：

TE [石油、天然气工业]; TK [能源与动力工程];

学科分类号：

0807 ; 0820 ;

摘要：

The green alga Nannochloropsis sp. was grown in a medium containing adequate (1.5 g/L sodium nitrate) and absence of nitrogen (0 g/L sodium nitrate) for improving the triacylglycerols (TAGs) level and fatty acid content for sustainable biodiesel production. Initially, the biomass yield of Nannochloropsis sp. was determined from the day of inoculation till 18th day and it is observed that on 14th day higher biomass yield at about 1.4 g/L was obtained. The Nile red stained cells from nitrogen sufficient and nitrogen starvation conditions observed under confocal microscopy revealed that neutral lipids exist as oil bodies were higher in nitrogen starved condition and also, the TAG content was 13.31% dryweight in nitrogen starved cells than nitrogen amended (10.46%) Nannochloropsis cells. The extracted lipids subjected for FTIR study showed the presence of TAG functional group in both condition and similarly, the FAME obtained from both condition showed the vibration at 1743 cm-1 for ester functional group. The fatty acid composition of nitrogen deplete condition revealed oleic acid methyl ester (28.7%), Behenic acid methyl ester (14.2%), cis-11, 14, 17-Eicosatrienoic acid methyl ester (8.8%) as predominant fatty acids than nitrogen supplied condition. The TAG content and FAME composition reported in this study makes Nannochloropsis sp. as plausible candidate for pilot scale biodiesel production.

引用

页数：7