Similar Features, Different Behaviors: A Comparative In Vitro Study of the Adipogenic Potential of Stem Cells from Human Follicle, Dental Pulp, and Periodontal Ligament

被引：14

作者：

Mercado-Rubio, Melissa D. ^{[1
]}

Perez-Argueta, Erick ^{[1
]}

Zepeda-Pedreguera, Alejandro ^{[1
]}

Aguilar-Ayala, Fernando J. ^{[2
]}

Penaloza-Cuevas, Ricardo ^{[2
]}

Ku-Gonzalez, Angela ^{[3
]}

Rojas-Herrera, Rafael A. ^{[1
]}

Rodas-Junco, Beatriz A. ^{[2
,4
]}

Nic-Can, Geovanny I. ^{[2
,4
]}

机构：

[1] Univ Autonoma Yucatan, Fac Ingn Quim, Perifer Norte Kilometro 33-5,Tablaje Catastral 13, Merida 97203, Yucatan, Mexico

[2] Univ Autonoma Yucatan, Lab Translac Celulas Troncales, Fac Odontol, Calle 61-A 10,Av Itzaes Costado Parque Paz,Col Ct, Merida 97000, Yucatan, Mexico

[3] Ctr Invest Cientif Yucatan, Unidad Bioquim & Biol Mol Plantas, Calle 43 130,Col Chuburna De Hidalgo, Merida 97200, Yucatan, Mexico

[4] Univ Autonoma Yucatan, CONACYT, Fac Ingn Quim, Perifer Norte Kilometro 33-5,Tablaje Catastral 13, Merida 97203, Yucatan, Mexico

来源：

JOURNAL OF PERSONALIZED MEDICINE | 2021年 / 11卷 / 08期

关键词：

adipogenesis; cellular differentiation; dental tissue-derived mesenchymal stem cells; dental pulp cells; dental follicle cells; periodontal ligament cells; DECIDUOUS TEETH; EXPRESSION; ADIPOCYTES; PROGENITORS; LINEAGE; SYSTEM; WHITE;

D O I：

10.3390/jpm11080738

中图分类号：

R19 [保健组织与事业（卫生事业管理）];

学科分类号：

摘要：

Dental tissue-derived mesenchymal stem cells (DT-MSCs) are a promising resource for tissue regeneration due to their multilineage potential. Despite accumulating data regarding the biology and differentiation potential of DT-MSCs, few studies have investigated their adipogenic capacity. In this study, we have investigated the mesenchymal features of dental pulp stem cells (DPSCs), as well as the in vitro effects of different adipogenic media on these cells, and compared them to those of periodontal ligament stem cells (PLSCs) and dental follicle stem cells (DFSCs). DFSC, PLSCs, and DPSCs exhibit similar morphology and proliferation capacity, but they differ in their self-renewal ability and expression of stemness markers (e.g OCT4 and c-MYC). Interestingly, DFSCs and PLSCs exhibited more lipid accumulation than DPSCs when induced to adipogenic differentiation. In addition, the mRNA levels of adipogenic markers (PPAR, LPL, and ADIPOQ) were significantly higher in DFSCs and PLSCs than in DPSCs, which could be related to the differences in the adipogenic commitment in those cells. These findings reveal that the adipogenic capacity differ among DT-MSCs, features that might be advantageous to increasing our understanding about the developmental origins and regulation of adipogenic commitment.

引用

页数：18

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