Increased Migration by Stimulation of Thymidine Phosphorylase in Endothelial Cells of Different Origin

被引：5

作者：

Bijnsdorp, I. V. ^{[1
]}

Vrijland, K. ^{[1
]}

Vroling, L. ^{[1
]}

Fukushima, M. ^{[2
]}

Peters, G. J. ^{[1
]}

机构：

[1] Vrije Univ Amsterdam, Med Ctr, Dept Med Oncol, NL-1007 MB Amsterdam, Netherlands

[2] Taiho Pharmaceut Co Ltd, Tokushima Res Inst, Tokushima, Japan

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2010年 / 29卷 / 4-6期

关键词：

HUVEC; ECFC; migration; invasion; thymidine phosphorylase; GROWTH FACTOR/THYMIDINE PHOSPHORYLASE; COLONY-FORMING CELLS; ANGIOGENIC FACTORS;

D O I：

10.1080/15257771003730201

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thymidine phosphorylase (TP) catalyzes the phosphorylytic cleavage of thymidine to thymine and deoxyribose-1-phosphate. The latter may be involved in the angiogenic stimulation of TP. In the present study, we investigated whether thymidine and deoxyribose (dR) could stimulate angiogenesis in vitro of two types of endothelial cells (isolated from umbilical veins (HUVEC) and endothelial colony forming cells (ECFC)), and whether the stereoisomer L-deoxyribose (L-dR) and the thymidine phosphorylase inhibitor (TPI) could reduce this. Both cell types had a low TP activity. Thymidine increased the migration of both HUVECs and ECFCs, but dR only that of the ECFCs. The invasion was not changed by any of the agents tested. In conclusion, TP may play a role in the migration of HUVECs and ECFCs, but not the invasion.

引用

页码：482 / 487

页数：6

共 17 条

[1] Isolation of putative progenitor endothelial cells for angiogenesis [J].

Asahara, T ;

Murohara, T ;

Sullivan, A ;

Silver, M ;

vanderZee, R ;

Li, T ;

Witzenbichler, B ;

Schatteman, G ;

Isner, JM .

SCIENCE, 1997, 275 (5302) :964-967

[2] Microtubule-targeting agents inhibit angiogenesis at subtoxic concentrations, a process associated with inhibition of Rac1 and Cdc42 activity and changes in the endothelial cytoskeleton [J].

Bijman, Marcel N. A. ;

van Nieuw Amerongen, Geerten P. ;

Laurens, Nancy ;

van Hinsbergh, Victor W. M. ;

Boven, Epie .

MOLECULAR CANCER THERAPEUTICS, 2006, 5 (09) :2348-2357

[3] The Dual Role of Thymidine Phosphorylase in Cancer Development and Chemotherapy [J].

Bronckaers, Annelies ;

Gago, Federico ;

Balzarini, Jan ;

Liekens, Sandra .

MEDICINAL RESEARCH REVIEWS, 2009, 29 (06) :903-953

[4]

Brown NS, 2000, CANCER RES, V60, P6298

[5]

Bruin M, 2006, CANC THERAPY, V4, P99

[6] Sulfasalazine down-regulates the expression of the angiogenic factors platelet-derived endothelial cell growth factor/thymidine phosphorylase and interleukin-8 in human monocytic-macrophage THP1 and U937 cells [J].

de Bruin, M ;

Peters, GJ ;

Oerlemans, R ;

Assaraf, YG ;

Masterson, AJ ;

Adema, AD ;

Dijkmans, BAC ;

Pinedo, HM ;

Jansen, G .

MOLECULAR PHARMACOLOGY, 2004, 66 (04) :1054-1060

[7] Role of platelet-derived enclothelial cell growth factor/thymidine phosphorylase in fluoropyrimidine sensitivity [J].

de Bruin, M ;

van Capel, T ;

Van der Born, K ;

Kruyt, FA ;

Fukushinna, M ;

Hoekman, K ;

Pinedo, HM ;

Peters, GJ .

BRITISH JOURNAL OF CANCER, 2003, 88 (06) :957-964

[8] Thymidine phosphorylase and 2-deoxyribose stimulate human endothelial cell migration by specific activation of the integrins α5β1 and αVβ3 [J].

Hotchkiss, KA ;

Ashton, AW ;

Schwartz, EL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (21) :19272-19279

[9]

Hotchkiss KA, 2003, CANCER RES, V63, P527

[10] Acute myocardial infarction in swine rapidly and selectively releases highly proliferative endothelial colony forming cells (ECFCs) into circulation [J].

Huang, Lan ;

Hou, Dongming ;

Thompson, Meredith A. ;

Baysden, Sarah E. ;

Shelley, W. Christopher ;

Ingram, David A. ;

March, Keith L. ;

Yoder, Mervin C. .

CELL TRANSPLANTATION, 2007, 16 (09) :887-897

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