Anaerotignum faecicola sp. nov., isolated from human faeces

A strictly anaerobic bacterium, designated as strain KGMB-03357(T), was isolated from the faeces of a healthy Korean selected by Bundang Seoul National University based on health status. Cells of strain KGMB03357(T) are Gram-stain-positive, non-motile, non-spore-forming, and observed as straight or curved rods. The isolate grew at 10-45 degrees C (optimum temperature of 40 degrees C) and a pH range of 5.1-10.5 (optimum pH of 6.8). Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357(T) forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17(T) (= KCTC 15066(T), 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582(T), 94.9%), Anaerotignum neopropionicum DSM 03847(T) (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021(T) (= KCTC 15705T, 94.8%). The ANI values between strain KGMB 03357T and members of the genus Anaerotignum were 73.3-71.0%, which are below the ANI criterion for interspecies identity. The DNA G + C content based on the whole-genome sequence is 47.3 mol%. The major cellular fatty acids of strain KGMB03357(T) are C-16:0, C-18:0, C-18:1 cis 9, and anteiso-C-15:0. Strain KGMB03357T contains meso-diaminopimelic acid as the diagnostic amino acid in the cell wall peptidoglycan. Based on the phenotypic, phylogenetic, and genomic properties, strain KGMB 03357(T) represents a novel species of the genus Anaerotignum, for which the name Anaerotignum faecicola sp. nov. is proposed. The type strain is KGMB03357(T) (= KCTC 15736T = DSM 107953(T)).