Analysis of chemical composition of polysaccharides from Poria cocos Wolf and its anti-tumor activity by NMR spectroscopy

被引：60

作者：

Ke RuiDian ^{[1
]}

Lin ShunFa ^{[1
]}

Chen Yi ^{[1
]}

Ji ChuRong ^{[2
]}

Shu QiaGuang ^{[3
]}

机构：

[1] Shantou Univ, Dept Radiol, Affiliated Hosp 1, Coll Med, Shantou 515041, Guangdong, Peoples R China

[2] Shantou Univ, Dept Obstet, Affiliated Hosp 1, Coll Med, Shantou 515041, Guangdong, Peoples R China

[3] Second People Hosp ZhongShan, Dept TB, Guangzhou, Guangdong, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2010年 / 80卷 / 01期

关键词：

Anti-tumor activity; Polysaccharide from Poria cocos Wolf; GC-MS; NMR spectroscopy; Chemical structure; ACTIVATION;

D O I：

10.1016/j.carbpol.2009.10.063

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this Study, we extracted the polysaccharide from Poria cocos Wolf and examined its chemical components using gas chromatography-mass spectrophotometer (GC-MS) and fourier transform infrared (FT-IR) spectra. Then its anti-tumor effects was investigated via in vivo assays. Chemical analysis revealed that the polysaccharide was composed of ribose, arabinose, xylose, mannose, glucose, and galactose in the molar contents of 1.49, 1.17, 0.62, 10.34, 86.39 and 1.31 mu M. respectively, and their corresponding mole percentages were 1.6%. 1.09%, 0.54%. 11.3%, 85.9%, and 1.01%, respectively. After 7 weeks of feeding, the polysaccharide significantly inhibited tumor cells growth and increased Serum Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities in rats. These results indicate that the polysaccharide possessed strong anti-tumor activity. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：31 / 34

页数：4

共 13 条

[1]

DUBOIS M, 1956, ANAL CHEM, V28, P356, DOI DOI 10.1021/AC60111A017

[2] Determining the degree of methylesterification of pectin by ATR/FT-IR: Methodology optimisation and comparison with theoretical calculations [J].

Fellah, Abdenor ;

Anjukandi, Padmesh ;

Waterland, Mark R. ;

Williams, Martin A. K. .

CARBOHYDRATE POLYMERS, 2009, 78 (04) :847-853

[3]

Hugo A., 1984, METHOD ENZYMOL, V105, P121, DOI [10.1016/S0076-6879, 10.1016/S0076-6879(84)05016-3, DOI 10.1016/S0076-6879(84)05016-3]

[4] Monitoring activation sites on polysaccharides by GC-MS [J].

Kim, John S. ;

Laskowich, Erin R. ;

Michon, Francis ;

Kaiser, Raymond E. ;

Arumugham, Rasappa G. .

ANALYTICAL BIOCHEMISTRY, 2006, 358 (01) :136-142

[5] Polysaccharide isolated from Poria cocos sclerotium induces NF-κB/Rel activation and iNOS expression in murine macrophages [J].

Lee, KY ;

Jeon, YJ .

INTERNATIONAL IMMUNOPHARMACOLOGY, 2003, 3 (10-11) :1353-1362

[6] Chemical characteristic and anticoagulant activity of the sulfated polysaccharide isolated from Monostroma latissimum (Chlorophyta) [J].

Mao, Wenjun ;

Li, Hongyan ;

Li, Yi ;

Zhang, Huijuan ;

Qi, Xiaohui ;

Sun, Haihong ;

Chen, Yin ;

Guo, Shoudong .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2009, 44 (01) :70-74

[7]

MARKLUND S, 1974, European Journal of Biochemistry, V47, P469, DOI 10.1111/j.1432-1033.1974.tb03714.x

[8] Effects of Hoelen on the efferent activity of the gastric vagus nerve in the rat [J].

Okui, Y ;

Morita, M ;

Iizuka, A ;

Komatsu, Y ;

Okada, M ;

Maruno, M ;

Niijima, A .

JAPANESE JOURNAL OF PHARMACOLOGY, 1996, 72 (01) :71-73

[9]

PAGLIA DE, 1967, J LAB CLIN MED, V70, P158

[10] Vibrational spectroscopy and X-ray diffraction methods to establish the differences between hardwood and softwood [J].

Popescu, Carmen-Mihaela ;

Singurel, Ghita ;

Popescu, Maria-Cristina ;

Vasile, Cornelia ;

Argyropoulos, Dimitris S. ;

Willfor, Stefan .

CARBOHYDRATE POLYMERS, 2009, 77 (04) :851-857

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