Flavihalobacter algicola gen. nov. sp. nov., a member of the family Flavobacteriaceae with alginate- degradation activity, isolated from marine alga Saccharina japonica

被引:3
作者
Yin, Rui [1 ]
Yi, Yan-Jun [1 ]
Chen, Zhuo [1 ]
Chen, Guan-Jun [1 ]
Zhou, Yan-Xia [1 ]
Du, Zong-Jun [1 ]
机构
[1] Shandong Univ, Marine Coll, Weihai 264209, Peoples R China
基金
中国博士后科学基金; 中国国家自然科学基金;
关键词
16S rRNA gene sequence; Flavihalobacter algicola; polyphasic taxonomy; EMENDED DESCRIPTION; WINOGRADSKYELLA-THALASSOCOLA; SECONDARY STRUCTURE; BROWN ALGA; BACTERIUM; BIZIONIA; GENOME; SEA; SEAWATER; ECHINORUM;
D O I
10.1099/ijsem.0.004701
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A Gram- stain-negative, aerobic, yellow, non-motile, rod-shaped and alginate-degrading bacterium, designated Dm15(T), was isolated from marine alga collected in Weihai, PR China. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Dm15(T) represents a distinct line of the family Flavobacteriaceae. Strain Dm15(T) had the highest 16S rRNA gene sequence similarity to its closest phylogenetic neighbour Arcticiflavibacter luteus (96.7 %) and 93.7?96.4 % sequence similarity to other phylogenetic neighbours (Bizionia paragorgiae, Winogradskyella thalassocola, Ichthyenterobacterium magnum, Psychroserpens burtonensis and Arcticiflavibacter luteus) in the family Flavobacteriaceae. The novel isolate was able to grow at 10?40 ?C (optimum, 30?33 degrees C), pH 7.0?9.0 (optimum, pH 7.0-7.5) and with 0.5-6.0 % NaCl (optimum 2.0-3.0 %, w/v). It could grow at 40 degrees C, and degrade alginate and cellulose, which were different from the neighbour genera. The draft genome consisted of 3395 genes with a total length of 3 798 431 bp and 34.1mol% G+C content. Especially, there were some specific genes coding for cellulase and alginate lyase, which provided a basis for the above phenotypic characteristics. The strain's genome sequence showed 71.1?80.2 % average amino acid identity values and 71.8?77.7 % average nucleotide identity values compared to the type strains of related genera within the family Flavobacteriaceae. It shared digital DNA?DNA hybridization identity of 19.8 and 20.9 % with I. magnum and A. luteus, respectively. The sole menaquinone was MK-6. The major fatty acids were iso- C15.0 and iso- C15.1 G. The polar lipids included six unidentified polar lipids, four unidentified aminolipids and phosphatidylethanolamine. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain Dm15T represents a novel species of a new genus in the family Flavobacteriaceae, phylum Bacteroidetes, for which the name Flavihalobacter algicola gen. nov., sp. nov. is proposed. The type strain is Dm15T (KCTC 42256T=CICC 23815T).
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页数:9
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共 58 条
  • [1] Dynamic programming algorithms for RNA secondary structure prediction with pseudoknots
    Akutsu, T
    [J]. DISCRETE APPLIED MATHEMATICS, 2000, 104 (1-3) : 45 - 62
  • [2] The RAST server: Rapid annotations using subsystems technology
    Aziz, Ramy K.
    Bartels, Daniela
    Best, Aaron A.
    DeJongh, Matthew
    Disz, Terrence
    Edwards, Robert A.
    Formsma, Kevin
    Gerdes, Svetlana
    Glass, Elizabeth M.
    Kubal, Michael
    Meyer, Folker
    Olsen, Gary J.
    Olson, Robert
    Osterman, Andrei L.
    Overbeek, Ross A.
    McNeil, Leslie K.
    Paarmann, Daniel
    Paczian, Tobias
    Parrello, Bruce
    Pusch, Gordon D.
    Reich, Claudia
    Stevens, Rick
    Vassieva, Olga
    Vonstein, Veronika
    Wilke, Andreas
    Zagnitko, Olga
    [J]. BMC GENOMICS, 2008, 9 (1)
  • [3] Psychroserpens jangbogonensis sp nov., a psychrophilic bacterium isolated from Antarctic marine sediment
    Baek, Kiwoon
    Lee, Yung Mi
    Hwang, Chung Yeon
    Park, Hyun
    Jung, You-Jung
    Kim, Mi-Kyeong
    Hong, Soon Gyu
    Kim, Ji Hee
    Lee, Hong Kum
    [J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2015, 65 : 183 - 188
  • [4] UniProt: a worldwide hub of protein knowledge
    Bateman, Alex
    Martin, Maria-Jesus
    Orchard, Sandra
    Magrane, Michele
    Alpi, Emanuele
    Bely, Benoit
    Bingley, Mark
    Britto, Ramona
    Bursteinas, Borisas
    Busiello, Gianluca
    Bye-A-Jee, Hema
    Da Silva, Alan
    De Giorgi, Maurizio
    Dogan, Tunca
    Castro, Leyla Garcia
    Garmiri, Penelope
    Georghiou, George
    Gonzales, Daniel
    Gonzales, Leonardo
    Hatton-Ellis, Emma
    Ignatchenko, Alexandr
    Ishtiaq, Rizwan
    Jokinen, Petteri
    Joshi, Vishal
    Jyothi, Dushyanth
    Lopez, Rodrigo
    Luo, Jie
    Lussi, Yvonne
    MacDougall, Alistair
    Madeira, Fabio
    Mahmoudy, Mahdi
    Menchi, Manuela
    Nightingale, Andrew
    Onwubiko, Joseph
    Palka, Barbara
    Pichler, Klemens
    Pundir, Sangya
    Qi, Guoying
    Raj, Shriya
    Renaux, Alexandre
    Lopez, Milagros Rodriguez
    Saidi, Rabie
    Sawford, Tony
    Shypitsyna, Aleksandra
    Speretta, Elena
    Turner, Edward
    Tyagi, Nidhi
    Vasudev, Preethi
    Volynkin, Vladimir
    Wardell, Tony
    [J]. NUCLEIC ACIDS RESEARCH, 2019, 47 (D1) : D506 - D515
  • [5] Bernardet J--F, 2012, BERGEYS MANUAL SYSTE, V4
  • [6] Cutting a gordian knot: Emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom nov (basonym, Cytophaga aquatilis Strohl and Tait 1978)
    Bernardet, JF
    Segers, P
    Vancanneyt, M
    Berthe, F
    Kersters, K
    Vandamme, P
    [J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1996, 46 (01): : 128 - 148
  • [7] Bernardet JF, 2002, INT J SYST EVOL MICR, V52, P1049, DOI [10.1099/ijs.0.02136-0, 10.1099/00207713-52-3-1049]
  • [8] antiSMASH 5.0: updates to the secondary metabolite genome mining pipeline
    Blin, Kai
    Shaw, Simon
    Steinke, Katharina
    Villebro, Rasmus
    Ziemert, Nadine
    Lee, Sang Yup
    Medema, Marnix H.
    Weber, Tilmann
    [J]. NUCLEIC ACIDS RESEARCH, 2019, 47 (W1) : W81 - W87
  • [9] A new method to predict the consensus secondary structure of a set of unaligned RNA sequences
    Bouthinon, D
    Soldano, H
    [J]. BIOINFORMATICS, 1999, 15 (10) : 785 - 798
  • [10] Psychroserpens burtonensis gen. nov, sp. nov, and Gelidibacter algens gen. nov, sp. nov, psychrophilic bacteria isolated from Antarctic lacustrine and sea ice habitats
    Bowman, JP
    McCammon, SA
    Brown, JL
    Nichols, PD
    McMeekin, TA
    [J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1997, 47 (03): : 670 - 677