Antifungal activity of tyrosol and farnesol used in combination against Candida species in the planktonic state or forming biofilms

被引：42

作者：

Monteiro, D. R. ^{[1
,2
]}

Arias, L. S. ^{[2
]}

Fernandes, R. A. ^{[2
,3
]}

Deszo da Silva, L. F. ^{[1
]}

de Castilho, M. O. V. F. ^{[1
]}

da Rosa, T. O. ^{[1
]}

Vieira, A. P. M. ^{[2
]}

Straioto, F. G. ^{[1
]}

Barbosa, D. B. ^{[3
]}

Delbem, A. C. B. ^{[2
]}

机构：

[1] Univ Western Sao Paulo UNOESTE, Grad Program Dent GPD Masters Degree, Rua Jose Bongiovani 700, BR-19050920 Presidente Prudente, SP, Brazil

[2] Sao Paulo State Univ Unesp, Dept Pediat Dent & Publ Hlth, Sch Dent, Sao Paulo, Brazil

[3] Sao Paulo State Univ Unesp, Dept Dent Mat & Prosthodont, Sch Dent, Sao Paulo, Brazil

来源：

JOURNAL OF APPLIED MICROBIOLOGY | 2017年 / 123卷 / 02期

关键词：

antimicrobials; biofilms; fungi; mycology; quorum sensing; AMPHOTERICIN-B; IN-VITRO; ALBICANS BIOFILMS; CHLORHEXIDINE; FLUCONAZOLE; EPIDERMIDIS; CASPOFUNGIN; MICAFUNGIN; VANCOMYCIN; EFFICACY;

D O I：

10.1111/jam.13513

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

AimThis study examined the antifungal activity of the combination of tyrosol and farnesol against Candida albicans and Candida glabrata in the planktonic state or forming biofilms. Methods and ResultsThe effect of drug association against Candida planktonic cells was assessed by the fractional inhibitory concentration index. Mono- and dual-species biofilms were developed during 24 h and then treated with the compounds for 3 days, with two daily treatments of 1 min each. After, the total biomass, metabolic activity and the number of cultivable cells were quantified. Planktonic cells of the two species showed a similar susceptibility to the drug combination, however, a synergistic effect was only verified for C. glabrata. Regarding biofilm susceptibility, significant reductions in C. glabrata biomass, metabolism of C. albicans and mixed biofilms, and cultivable cells of single biofilms were verified for the drug combination, indicating an additive effect. For all other experiments, the effects were classified as indifferent. ConclusionThe combined use of tyrosol and farnesol was advantageous for some of the analysed parameters against Candida species. Significance and Impact of the StudyThese findings may contribute to the development of oral care products containing tyrosol and farnesol to combat oral infections caused by Candida species.

引用

页码：392 / 400

页数：9

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