Electron tomography reveals the endoplasmic reticulum as a membrane source for autophagosome formation

被引：60

作者：

Hayashi-Nishino, Mitsuko ^{[1
,2
]}

Fujita, Naonobu ^{[3
]}

Noda, Takeshi ^{[3
]}

Yamaguchi, Akihito ^{[2
]}

Yoshimori, Tamotsu ^{[3
]}

Yamamoto, Akitsugu ^{[1
]}

机构：

[1] Nagahama Inst Biosci & Technol, Fac Biosci, Shiga, Japan

[2] Osaka Univ, Dept Cell Membrane Biol, Inst Sci & Ind Res, Osaka, Japan

[3] Osaka Univ, Res Inst Microbial Dis, Dept Cellular Regulat, Osaka, Japan

来源：

AUTOPHAGY | 2010年 / 6卷 / 02期

关键词：

ATG4B mutant; autophagosome; endoplasmic reticulum; electron tomography;

D O I：

10.4161/auto.6.2.11134

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The origin and source of autophagosomal membranes are long-standing questions. By electron microscopy, we show that the endoplasmic reticulum (ER) associates with early autophagic structures called isolation membranes (IM) or phagophores in mammalian culture cells. Overexpression of a mutant of Atg4B, which causes defects in autophagosome formation, caused accumulation of ER-IM complexes. Electron tomography revealed the ER-IM complex as a subdomain of the ER forming a cradle encircling the IM, and showed that both ER and isolation membranes are interconnected.

引用

页码：301 / 303

页数：3