Development and characterization of novel tetra-, tri- and di-nucleotide microsatellite markers in cacao (Theobroma cacao L.)

被引:7
作者
Araujo, Iona S.
Intorne, Aline C.
Pereira, Messias G.
Lopes, Uilson V.
de Souza Filho, Goncalo A. [1 ]
机构
[1] Univ Estadual Norte Fluminense, Lab Biotecnol, BR-28013600 Campos Dos Goytacazes, RJ, Brazil
[2] Univ Estadual Norte Fluminense, Lab Melhoramento Genet Vegetal, BR-28013600 Campos Dos Goytacazes, RJ, Brazil
[3] Ctr Pesquisas Cacau, CEPLAC, BR-45600000 Itabuna, BA, Brazil
关键词
microsatellite; molecular markers; primers; theobroma;
D O I
10.1007/s11032-006-9057-7
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The cacao plant, Theobroma cacao L., produces white seeds (beans) that form the major ingredient of processed chocolate. A great deal of research effort has been expended to the development of new genetically modified cacao plants with improved productivity and resistance and beans of good industrial quality. The availability of suitable genetic markers is an important aspect of the efficient selection and breeding of this perennial species. We describe the development of 123 microsatellite loci of cacao. An optimized protocol was used to construct and screen a microsatellite-enriched genomic library from which we isolated 64 di-nucleotide, 45 tri-nucleotide and 14 tetra-nucleotide microsatellite loci. The primers were tested on samples from five different T. cacao accessions, one accession from T. grandiflorum and one accession from Herranea sp. Among the 123 loci, 54 were polymorphic, 61 were monomorphic and eight did not present an amplification product. These new markers will be useful in future studies by increasing the accuracy of genotypic assessments in diverse cocoa tree populations as well as in other species of the Theobroma genus.
引用
收藏
页码:73 / 81
页数:9
相关论文
共 8 条
[1]   The use of self-pollinated progenies as 'in-groups' for the genetic characterization of cocoa germplasm [J].
Charters, YM ;
Wilkinson, MJ .
THEORETICAL AND APPLIED GENETICS, 2000, 100 (01) :160-166
[2]  
Faleiro F.G., 2002, AGROTROPICA, V14, P31
[3]  
FALEIRO FG, 2004, CROP BREED APPL BIOT, V4, P227
[4]  
Figueira AVO, 2001, MELHORAMENTO GENETIC, P385
[5]   Isolation and characterization of microsatellites in Theobroma cacao L. [J].
Lanaud, C ;
Risterucci, AM ;
Pieretti, I ;
Falque, M ;
Bouet, A ;
Lagoda, PJL .
MOLECULAR ECOLOGY, 1999, 8 (12) :2141-2143
[6]   Development and characterization of a large set of microsatellite markers in grapevine (Vitis vinifera L.) suitable for multiplex PCR [J].
Merdinoglu, D ;
Butterlin, G ;
Bevilacqua, L ;
Chiquet, V ;
Adam-Blondon, AF ;
Decroocq, S .
MOLECULAR BREEDING, 2005, 15 (04) :349-366
[7]   A new cacao linkage map based on codominant markers: development and integration of 201 new microsatellite markers [J].
Pugh, T ;
Fouet, O ;
Risterucci, AM ;
Brottier, P ;
Abouladze, M ;
Deletrez, C ;
Courtois, B ;
Clement, D ;
Larmande, P ;
N'Goran, JAK ;
Lanaud, C .
THEORETICAL AND APPLIED GENETICS, 2004, 108 (06) :1151-1161
[8]   Selection of international molecular standards for DNA fingerprinting of Theobroma cacao [J].
Saunders, JA ;
Mischke, S ;
Leamy, EA ;
Hemeida, AA .
THEORETICAL AND APPLIED GENETICS, 2004, 110 (01) :41-47