Detection of Japanese eel endothelial cells-infecting virus in Anguilla japonica elvers

被引:10
作者
Okazaki, Sachiko [1 ,2 ]
Yasumoto, Shinya [3 ]
Koyama, Satoshi [1 ,4 ]
Tsuchiaka, Shinobu [1 ,2 ]
Naoi, Yuki [2 ]
Omatsu, Tsutomu [1 ,2 ]
Ono, Shin-ichi [5 ]
Mizutani, Tetsuya [1 ,2 ]
机构
[1] Gifu Univ, United Grad Sch Vet Sci, 1-1 Yanagito, Gifu, Gifu 5011193, Japan
[2] Tokyo Univ Agr & Technol, Res & Educ Ctr Prevent Global Infect Dis Anim, 3-5-8 Saiwai Cho, Fuchu, Tokyo 1830054, Japan
[3] Natl Fisheries Univ, Dept Appl Aquabiol, 2-7-1 Nagata Honmachi, Shimonoseki, Yamaguchi 7596595, Japan
[4] Tokyo Univ Agr & Technol, Grad Sch Agr, 3-5-8 Saiwai Cho, Fuchu, Tokyo 1830054, Japan
[5] Tokai Univ, Sch Marine Sci & Technol, Shimizu Ku, 3-20-1 Orido, Shizuoka 4240902, Japan
关键词
Anguilla japonica; elver; Japanese eel endothelial cells-infecting virus; TRANSPLACENTAL TRANSMISSION; INTENSE CONGESTION; POLYOMA-VIRUS; GILLS; WILD; BK;
D O I
10.1292/jvms.15-0515
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Japanese eel endothelial cells-infecting virus (JEECV) has spread in eel farms and caused serious economic loss. In this study, we examined the prevalence of JEECV infection in 100 wild Japanese eel (Anguilla japonica) elvers caught from Yamaguchi prefecture, Japan, using quantitative PCR and conventional PCR. Total genomic DNA was obtained from the cranial quarter of the body in 70 of 100 eels and from the gill in the remaining. Of 30 gill samples, 20 were analyzed after pooling with other samples, and the remaining 10 were analyzed separately. A single positive result for JEECV was detected following analysis of the 10 separately analyzed samples. This result constitutes the first report of JEECV infection in wild A. japonica elvers.
引用
收藏
页码:705 / 707
页数:3
相关论文
共 21 条
[1]   Silver European eels health in Mediterranean habitats [J].
Amilhat, Elsa ;
Fazio, Geraldine ;
Simon, Gael ;
Manetti, Marc ;
Paris, Severine ;
Delahaut, Laurence ;
Farrugio, Henri ;
Lecomte-Finiger, Raymonde ;
Sasal, Pierre ;
Faliex, Elisabeth .
ECOLOGY OF FRESHWATER FISH, 2014, 23 (01) :49-64
[2]   Serological evidence of vertical transmission of JC and BK polyomaviruses in humans [J].
Boldorini, Renzo ;
Allegrini, Sara ;
Miglio, Umberto ;
Paganotti, Alessia ;
Cocca, Norma ;
Zaffaroni, Mauro ;
Riboni, Francesca ;
Monga, Guido ;
Viscidi, Raphael .
JOURNAL OF GENERAL VIROLOGY, 2011, 92 :1044-1050
[3]  
BRANDNER G, 1977, J CLIN MICROBIOL, V5, P250
[4]   HISTOPATHOLOGICAL OBSERVATIONS ON AN INTENSE CONGESTION OF THE GILLS IN CULTURED JAPANESE EEL, ANGUILLA-JAPONICA [J].
EGUSA, S ;
TANAKA, M ;
OGAMI, H ;
OKA, H .
FISH PATHOLOGY, 1989, 24 (01) :51-56
[5]  
ICES, 2006, EIFAC OCCASIONAL PAP, V38, P352
[6]   A HISTOPATHOLOGICAL STUDY ON THE ETIOLOGY OF INTENSE CONGESTION OF THE GILLS OF JAPANESE EEL, ANGUILLA-JAPONICA [J].
INOUYE, K ;
MIWA, S ;
AOSHIMA, H ;
OKA, H ;
SORIMACHI, M .
FISH PATHOLOGY, 1994, 29 (01) :35-41
[7]   THE OCCURRENCE OF VIRUS-INFECTIONS IN ELVERS AND EELS (ANGUILLA-ANGUILLA) IN EUROPE WITH PARTICULAR REFERENCE TO VHSV AND IHNV [J].
JORGENSEN, PEV ;
CASTRIC, J ;
HILL, B ;
LJUNGBERG, O ;
DEKINKELIN, P .
AQUACULTURE, 1994, 123 (1-2) :11-19
[8]  
Kusuda R., 1989, SUISANZOSHOKU, V37, P43
[9]   Novel DNA virus isolated from samples showing endothelial cell necrosis in the Japanese eel, Anguilla japonica [J].
Mizutani, Tetsuya ;
Sayama, Yusuke ;
Nakanishi, Akira ;
Ochiai, Hideharu ;
Sakai, Kouji ;
Wakabayashi, Kouji ;
Tanaka, Nozomi ;
Miura, Emi ;
Oba, Mami ;
Kurane, Ichiro ;
Saijo, Masayuki ;
Morikawa, Shigeru ;
Ono, Shin-ichi .
VIROLOGY, 2011, 412 (01) :179-187
[10]  
Okazaki S., 2014, J FISH DIS, V13, P12294