In vitro metabolism of the glycosidic sweeteners, stevia mixture and enzymatically modified stevia in human intestinal microflora

被引:153
作者
Koyama, E
Kitazawa, K
Ohori, Y
Izawaa, O
Kakegawa, K
Fujino, A
Ui, M
机构
[1] Mitsubishi Chem Safety Inst Ltd, Kashima Lab, Dept Biol, Hasaki, Ibaraki 3140255, Japan
[2] Maruzen Pharmaceut Co, Stevia Ind Assoc, Tokyo, Japan
[3] Tokyo Metropolitan Inst Med Sci, Tokyo 113, Japan
关键词
stevia mixture; enzymatically modified stevia; steviol; LC/ESI/MS; human intestinal microflora;
D O I
10.1016/S0278-6915(02)00235-1
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Stevia mixture, sweeteners extracted from the leaves of Stevia rebaudiana Bertom, consists mainly of stevioside and rebaudioside A (glycosides of the diterpene derivative steviol). The aim of this study was to investigate human intestinal metabolism of stevia mixture and its alpha-glucose derivative (known in Japan as enzymatically modified stevia) by LC/MS/ESI analysis. Degradation was examined by incubating stevia mixture, enzymatically modified stevia, stevioside, rebaudioside A, alpha-monoglucosylstevioside, alpha-monoglucosylrebaudioside A and the aglycone, steviol with pooled human faecal homogenates (obtained from five healthy volunteers) for 0, 8 and 24 h under anaerobic conditions. Stevia mixture, enzymatically modified stevia, stevioside and rebaudioside A (0.2 mg/ml) were completely eliminated within 24 h, whereas no degradation of steviol (0.08 and 0.2 mg/ml) appeared to be found during the incubation period. Stevia mixture, stevioside and rebaudioside A appeared to be hydrolyzed to steviol by human intestinal microflora: this observation is consistent with previous rat metabolism studies. Similarly, enzymatically modified stevia appeared to be metabolized via stevia components and, finally, to steviol. This study suggests that there are apparently no species differences in intestinal metabolism of stevia mixture between rats and humans. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:359 / 374
页数:16
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