Imaging of the glial activation that occurs in response to central nervous system trauma and inflammation could become a powerful technique for the assessment of several neuropathologies. The selective uptake and metabolism of 2-F-18-fluoroacetate (F-18-FAC) in glia may represent an attractive strategy for imaging glial metabolism. Methods: We have evaluated the use of F-18-FAC as a specific PET tracer of glial cell metabolism in rodent models of glioblastoma, stroke, and ischemia-hypoxia. Results: Enhanced uptake of F-18-FAC was observed (6.98 +/- 0.43 percentage injected dose per gram [%ID/g]; tumor-to-normal ratio, 1.40) in orthotopic U87 xenografts, compared with healthy brain tissue. The lesion extent determined by F-18-FAC PET correlated with that determined by MRI (R-2 = 0.934, P = 0.007). After transient middle cerebral artery occlusion in the rat brain, elevated uptake of F-18-FAC (1.00 +/- 0.03 % ID/g; lesion-to-normal ratio, 1.90) depicted the ischemic territory and correlated with infarct volumes as determined by 2,3,5-triphenyltetrazolium chloride staining (R-2 = 0.692, P = 0.010) and with the presence of activated astrocytes detected by anti-glial fibrillary acidic protein. Ischemia-hypoxia, induced by permanent ligation of the common carotid artery with transient hypoxia, resulted in persistent elevation of F-18-FAC uptake within 30 min of the induction of hypoxia. Conclusion: Our data support the further evaluation of F-18-FAC PET for the assessment of glial cell metabolism associated with neuroinflammation.
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Department of Diagnostic Radiology and Nuclear Medicine, Gunma University Graduate School of Medicine, Maebashi 371-8511Department of Diagnostic Radiology and Nuclear Medicine, Gunma University Graduate School of Medicine, Maebashi 371-8511
Endo K.
Oriuchi N.
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Department of Diagnostic Radiology and Nuclear Medicine, Gunma University Graduate School of Medicine, Maebashi 371-8511Department of Diagnostic Radiology and Nuclear Medicine, Gunma University Graduate School of Medicine, Maebashi 371-8511
Oriuchi N.
Higuchi T.
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Department of Diagnostic Radiology and Nuclear Medicine, Gunma University Graduate School of Medicine, Maebashi 371-8511Department of Diagnostic Radiology and Nuclear Medicine, Gunma University Graduate School of Medicine, Maebashi 371-8511
Higuchi T.
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Iida Y.
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Hanaoka H.
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Miyakubo M.
Ishikita T.
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Department of Diagnostic Radiology and Nuclear Medicine, Gunma University Graduate School of Medicine, Maebashi 371-8511Department of Diagnostic Radiology and Nuclear Medicine, Gunma University Graduate School of Medicine, Maebashi 371-8511
Ishikita T.
Koyama K.
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Department of Diagnostic Radiology, Gunma Prefectural Cardiovascular Center, MaebashiDepartment of Diagnostic Radiology and Nuclear Medicine, Gunma University Graduate School of Medicine, Maebashi 371-8511
机构:
Korea Inst Radiol & Med Sci, Div RI Convergence Res, Seoul 01812, South KoreaKorea Inst Radiol & Med Sci, Div RI Convergence Res, Seoul 01812, South Korea
Woo, Sang-Keun
Moon, Byung Seok
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Seoul Natl Univ, Coll Med, Dept Nucl Med, Bundang Hosp, Seongnam 13620, South KoreaKorea Inst Radiol & Med Sci, Div RI Convergence Res, Seoul 01812, South Korea
Moon, Byung Seok
Kim, Bom Sahn
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Ewha Womans Univ, Dept Nucl Med, Sch Med, Seoul 07985, South KoreaKorea Inst Radiol & Med Sci, Div RI Convergence Res, Seoul 01812, South Korea
Kim, Bom Sahn
Kim, Min Hwan
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Korea Inst Radiol & Med Sci, Div RI Convergence Res, Seoul 01812, South KoreaKorea Inst Radiol & Med Sci, Div RI Convergence Res, Seoul 01812, South Korea
Kim, Min Hwan
Lee, Yong Jin
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Korea Inst Radiol & Med Sci, Div RI Convergence Res, Seoul 01812, South KoreaKorea Inst Radiol & Med Sci, Div RI Convergence Res, Seoul 01812, South Korea
Lee, Yong Jin
Jung, Jae Ho
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Seoul Natl Univ, Coll Med, Dept Nucl Med, Bundang Hosp, Seongnam 13620, South KoreaKorea Inst Radiol & Med Sci, Div RI Convergence Res, Seoul 01812, South Korea
Jung, Jae Ho
Lee, Kyo Chul
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Korea Inst Radiol & Med Sci, Div RI Convergence Res, Seoul 01812, South KoreaKorea Inst Radiol & Med Sci, Div RI Convergence Res, Seoul 01812, South Korea
Lee, Kyo Chul
Seo, Youngho
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Univ Calif San Francisco, Sch Med, Dept Radiol, San Francisco, CA 94143 USAKorea Inst Radiol & Med Sci, Div RI Convergence Res, Seoul 01812, South Korea
Seo, Youngho
Kim, Wook
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Korea Inst Radiol & Med Sci, Div RI Convergence Res, Seoul 01812, South KoreaKorea Inst Radiol & Med Sci, Div RI Convergence Res, Seoul 01812, South Korea
Kim, Wook
Lim, Sang Moo
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Korea Inst Radiol & Med Sci, Div RI Convergence Res, Seoul 01812, South KoreaKorea Inst Radiol & Med Sci, Div RI Convergence Res, Seoul 01812, South Korea
Lim, Sang Moo
Lee, Byung Chul
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Seoul Natl Univ, Coll Med, Dept Nucl Med, Bundang Hosp, Seongnam 13620, South Korea
Ctr Nanomol Imaging & Innovat Drug Dev, Adv Inst Convergence Technol, Suwon 16229, South KoreaKorea Inst Radiol & Med Sci, Div RI Convergence Res, Seoul 01812, South Korea
Lee, Byung Chul
Kim, Sang Eun
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Seoul Natl Univ, Coll Med, Dept Nucl Med, Bundang Hosp, Seongnam 13620, South Korea
Ctr Nanomol Imaging & Innovat Drug Dev, Adv Inst Convergence Technol, Suwon 16229, South Korea
Seoul Natl Univ, Grad Sch Convergence Sci & Technol, Dept Transdisciplinary Studies, Seoul 08826, South KoreaKorea Inst Radiol & Med Sci, Div RI Convergence Res, Seoul 01812, South Korea