A newly identified polyunsaturated macamide alleviates dextran sulfate sodium-induced colitis in mice

被引：8

作者：

Zha, Rui ^{[1
]}

Ge, Enhui ^{[1
]}

Guo, Lirong ^{[1
,2
]}

Gao, Qing ^{[1
,2
]}

Lin, Qiqi ^{[1
]}

Zhou, Wan ^{[1
,2
]}

Jin, Xiaobao ^{[1
]}

Xie, Weiquan ^{[3
,4
]}

Yin, Hui ^{[1
]}

Liu, Tao ^{[1
]}

机构：

[1] Guangdong Pharmaceut Univ, Sch Biosci & Biopharmaceut, Guangdong Prov Key Lab Pharmaceut Bioact Subst, Guangzhou 510006, Peoples R China

[2] Guangdong Pharmaceut Univ, Sch Pharm, Guangzhou 510006, Peoples R China

[3] Univ South China, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China

[4] Guilin Med Univ, Sch Pharm, Guilin 541001, Peoples R China

来源：

FITOTERAPIA | 2021年 / 152卷

基金：

中国国家自然科学基金;

关键词：

Macamide; Docosahexaenoic acid; Ulcerative colitis; Cytokine; Tight junction; N-Benzyl docosahexaenamide; INFLAMMATORY BOWEL DISEASES; LEPIDIUM-MEYENII; TIGHT JUNCTIONS; THERAPY; HEALTH;

D O I：

10.1016/j.fitote.2021.104916

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Macamides are a class of bioactive amide alkaloids found only in maca (Lepidium meyenii). Recent studies have shown that macamide-rich extracts possess various biological activities, such as antioxidative, immuneenhancing, and reproductive health-improving activities. In the present study, N-benzyl docosahexaenamide (NB-DHA), a newly identified macamide with the highest degree of unsaturation among all identified macamides, was identified from the maca extract. Microalgae oil, a docosahexaenoic acid-rich substance, was used as the starting material for the synthesis of NB-DHA. The effects of NB-DHA in colitis-induced mice were evaluated. NB-DHA significantly alleviated weight loss, shortening of colon length, and occult blood occurrence in mice with dextran sulfate sodium-induced colitis. Histological analysis revealed that following the administration of NB-DHA in mice with colitis, the infiltration of inflammatory cells and levels of proinflammatory factors, such as tumor necrosis factor-alpha, interleukin (IL)-1 beta, IL-6, and myeloperoxidase, decreased, whereas the level of the antiinflammatory factor IL-10 increased. Furthermore, the decreased expression of intestinal tight junction proteins caused by colitis was upregulated by the administration of NB-DHA. These results indicate that NB-DHA could be developed as a therapeutic agent for ulcerative colitis.

引用

页数：9