Laser-diode-stimulated emission depletion microscopy

被引：51
|
作者：
Westphal, V [1 ]
Blanca, CM [1 ]
Dyba, M [1 ]
Kastrup, L [1 ]
Hell, SW [1 ]
机构：
[1] Max Planck Inst Biophys Chem, Dept Nanobiophoton, Gottingen, Germany
来源：
APPLIED PHYSICS LETTERS | 2003年 / 82卷 / 18期
关键词：
D O I：
10.1063/1.1571656
中图分类号：
O59 [应用物理学];
学科分类号：
摘要：
We report subdiffraction resolution in far-field fluorescence microscopy through laser-diode-stimulated emission depletion of molecular markers. The diode-generated focal intensities lead to a resolution improvement by similar to45% in both lateral directions. Excitation and stimulated emission are performed with electronically synchronized diode pulses of 50-70 ps and 300-400 ps duration, respectively. The subdiffraction resolution is utilized to resolve neighboring individual molecules. (C) 2003 American Institute of Physics.
引用
收藏
页码：3125 / 3127
页数：3
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