Individual heterogeneity screened umbilical cord-derived mesenchymal stromal cells with high Treg promotion demonstrate improved recovery of mouse liver fibrosis

被引：24
作者：
Xie, Yuanyuan [1 ]
Liu, Shuo [1 ]
Wang, Liudi [1 ]
Yang, Hui [1 ]
Tai, Chenxu [1 ]
Ling, Li [2 ,3 ]
Chen, Libo [2 ,3 ]
Liu, Shanshan [4 ]
Wang, Bin [1 ]
机构：
[1] Nanjing Univ, Med Sch, Affiliated Drum Tower Hosp, Clin Stem Cell Ctr, 321 Zhongshan Rd, Nanjing 210000, Peoples R China
[2] Hua Zhong Univ Sci & Technol, Union Shenzhen Hosp, Univ Hlth Sci Ctr, Dept Endocrinol, Shenzhen 518052, Guangdong, Peoples R China
[3] 6th Affiliated Hosp Shenzhen, Shenzhen 518052, Guangdong, Peoples R China
[4] Nanjing Univ, Affiliated Drum Tower Hosp, Dept Rheumatol & Immunol, Med Sch, Nanjing 210000, Peoples R China
来源：
STEM CELL RESEARCH & THERAPY | 2021年 / 12卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Cell therapy; Umbilical cord mesenchymal stromal cells; Heterogeneity; Differentiation; Immunomodulation; Liver fibrosis; STEM-CELLS; BONE; DIFFERENTIATION; THERAPY; PROLIFERATION; IMMUNOMODULATION; TRANSPLANTATION; OSTEOGENESIS; MECHANISMS; DISEASES;
D O I：
10.1186/s13287-021-02430-6
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Background To investigate the heterogeneities of human umbilical cord mesenchymal stromal cells (HUCMSCs) derived from different donors and their therapeutic variations when applied to mouse liver fibrosis model. Methods The characteristics of HUCMSCs derived from multiple donors were comprehensively analyzed including expressions of surface markers, viability, growth curve, karyotype analysis, tumorigenicity, differentiation potentials, and immune regulation capability. Then, the HUCMSCs with distinct immunomodulatory effects were applied to treat mouse liver fibrosis and their therapeutic effects were observed. Results The HUCMSCs derived from multiple donors kept a high consistency in surface marker expressions, viability, growth curve, and tumorigenicity in nude mice but had robust heterogeneities in differentiation potentials and immune regulations. In addition, three HUCMSC lines applied to mice liver fibrosis model had different therapeutic outcomes, in line with individual immune regulation capability. Conclusion The HUCMSCs derived from different donors have individual heterogeneity, which potentially lead to distinct therapeutic outcomes in mouse liver fibrosis, indicating we could make use of the donor-variation of MSCs to screen out guaranteed general indicators of MSCs for specific diseases in further stromal cell therapy.
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页数：17
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