The acid soluble extracellular polymeric substance of aerobic granular sludge dominated by Defluviicoccus sp.

被引：75
作者：
Pronk, M. [1 ]
Neu, T. R. [2 ]
van Loosdrecht, M. C. M. [1 ]
Lin, Y. M. [1 ]
机构：
[1] Delft Univ Technol, Dept Biotechnol, Maasweg 9, NL-2629 HZ Delft, Netherlands
[2] UFZ, Helmholtz Ctr Environm Res, Dept River Ecol, Microbiol Interfaces, Brueckstr 3A, D-39114 Magdeburg, Germany
来源：
WATER RESEARCH | 2017年 / 122卷
关键词：
Defluviicoccus; Aerobic granular sludge; Extracellular polymeric substances; Biofilm; EPS extraction; GLYCOGEN-ACCUMULATING ORGANISMS; STRUCTURAL-ANALYSIS; ACTIVATED-SLUDGE; EXOPOLYSACCHARIDES; EXTRACTION; EPS; ALPHAPROTEOBACTERIA; REMOVAL; BIOFILM; RNA;
D O I：
10.1016/j.watres.2017.05.068
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
A new acid soluble extracellular polymeric substance (acid soluble EPS) was extracted from an acetate fed aerobic granular sludge reactor operated at 35 degrees C. Acid soluble EPS dominated granules exhibited a remarkable and distinctive tangled tubular morphology. These granules are dominated by Defluviicoccus Cluster II organisms. Acetic acid instead of the usually required alkaline extraction medium was needed to dissolve the granules and solubilise the polymeric matrix. The extracted acid soluble EPS was analysed and identified using various instrumental analysis including H-1 and C-13 Nuclear Magnetic Resonance, Fourier Transform Infrared Spectroscopy and Raman spectroscopy. In addition, the glycoconjugates were characterized by fluorescence lectin-binding analysis. The acid soluble EPS is alpha-(1 -> 4) linked polysaccharide, containing both glucose and galactose as monomers. There are -OCH3 groups connected to the glucose monomer. Transmission and scanning electron microscopy (TEM, SEM) as well as confocal laser scanning microscopy (CLSM) showed that the acid soluble EPS was present as a tightly bound capsular EPS around bacterial cells ordered into a sarcinae-like growth pattern. The special granule morphology is decided by the acid soluble EPS produced by Defluviicoccus Cluster II organisms. This work shows that no single one method can be used to extract all possible extracellular polymeric substances. Results obtained here can support the elucidation of biofilm formation and structure in future research. (C) 2017 The Authors. Published by Elsevier Ltd.
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页码：148 / 158
页数：11
相关论文
共 46 条
[11]  
Engelsen S. B., 2016, RAMAN SPECTRA CARBOH
[12]  
Felz S., 2016, JOVE-J VIS EXP, V2016
[13]   The biofilm matrix [J].
Flemming, Hans-Curt ;
Wingender, Jost .
NATURE REVIEWS MICROBIOLOGY, 2010, 8 (09) :623-633
[14]   Advancements in the application of aerobic granular biomass technology for sustainable treatment of wastewater [J].
Giesen, A. ;
de Bruin, L. M. M. ;
Niermans, R. P. ;
van der Roest, H. F. .
WATER PRACTICE AND TECHNOLOGY, 2013, 8 (01) :47-54
[15]  
KREISL P, 1986, SYST APPL MICROBIOL, V7, P293
[16]   Micro-scale observations of the structure of aerobic microbial granules used for the treatment of nutrient-rich industrial wastewater [J].
Lemaire, Romain ;
Webb, Richard I. ;
Yuan, Zhiguo .
ISME JOURNAL, 2008, 2 (05) :528-541
[17]   The chemical and mechanical differences between alginate-like exopolysaccharides isolated from aerobic flocculent sludge and aerobic granular sludge [J].
Lin, Y. M. ;
Sharma, P. K. ;
van Loosdrecht, M. C. M. .
WATER RESEARCH, 2013, 47 (01) :57-65
[18]   Characterization of alginate-like exopolysaccharides isolated from aerobic granular sludge in pilot-plant [J].
Lin, Yuemei ;
de Kreuk, Merle ;
van Loosdrecht, M. C. M. ;
Adin, Avner .
WATER RESEARCH, 2010, 44 (11) :3355-3364
[19]   Extraction of extracellular polymeric substances (EPS) of sludges [J].
Liu, H ;
Fang, HHP .
JOURNAL OF BIOTECHNOLOGY, 2002, 95 (03) :249-256
[20]   Optimization of operation conditions for the startup of aerobic granular sludge reactors biologically removing carbon, nitrogen, and phosphorous [J].
Lochmatter, Samuel ;
Holliger, Christof .
WATER RESEARCH, 2014, 59 :58-70
12345