The beneficial effects of a gas-permeable flask for expansion of Tumor-Infiltrating lymphocytes as reflected in their mitochondrial function and respiration capacity

被引：20

作者：

Forget, Marie-Andree ^{[1
]}

Haymaker, Cara ^{[1
]}

Dennison, Jennifer B. ^{[2
]}

Toth, Christopher ^{[1
]}

Maiti, Sourindra ^{[3
]}

Fulbright, Orenthial J. ^{[1
]}

Cooper, Laurence J. N. ^{[3
]}

Hwu, Patrick ^{[1
]}

Radvanyi, Laszlo G. ^{[1
,4
,5
]}

Bernatchez, Chantale ^{[1
]}

机构：

[1] Univ Texas MD Anderson Canc Ctr MDACC, Dept Melanoma Med Oncol, Houston, TX USA

[2] Univ Texas MDACC, Dept Syst Biol, Houston, TX USA

[3] Univ Texas MDACC, Dept Pediat, Houston, TX USA

[4] Lion Biotechnol, Tampa, FL USA

[5] Univ S Florida, H Lee Moffitt Canc Ctr, Dept Immunol, Tampa, FL 33682 USA

来源：

ONCOIMMUNOLOGY | 2016年 / 5卷 / 02期

基金：

美国国家卫生研究院;

关键词：

TIL ACT; gas-permeable membrane; mitochondrial function; oxidative phenotype; tumor-infiltrating lymphocytes; METASTATIC MELANOMA PATIENTS; ANTIGEN-PRESENTING CELLS; T-CELLS; ADOPTIVE IMMUNOTHERAPY; CHEMOTHERAPY; CANCER; MEMORY;

D O I：

10.1080/2162402X.2015.1057386

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Adoptive transfer of autologous ex vivo expanded tumor-infiltrating lymphocytes (TIL) is a highly successful cell therapy approach in the treatment of late-stage melanoma. Notwithstanding the success of this therapy, only very few centers worldwide can provide it. To make this therapy broadly available, one of the major obstacles to overcome is the complexity of culturing the TIL. Recently, major efforts have been deployed to resolve this issue. The use of the Gas-permeable flask (G-Rex) during the REP has been one application that has facilitated this process. Here we show that the use of this new device is able to rescue poor TIL growth and maintain clonal diversity while supporting an improved mitochondrial function.

引用

页数：7

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