A novel chimeric amine dehydrogenase shows altered substrate specificity compared to its parent enzymes

被引：87

作者：

Bommarius, Bettina R. ^{[1
]}

Schuermann, Martin ^{[2
]}

Bommarius, Andreas S. ^{[1
,3
]}

机构：

[1] Georgia Inst Technol, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA

[2] DSM Innovat Synth BV, NL-6160 MD Geleen, Netherlands

[3] Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA

来源：

CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 95期

基金：

美国国家科学基金会;

关键词：

PHENYLALANINE DEHYDROGENASE; LEUCINE DEHYDROGENASE; CHIRAL AMINES; P450;

D O I：

10.1039/c4cc06527a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We created a novel chimeric amine dehydrogenase (AmDH) via domain shuffling of two parent AmDHs ('L- and F-AmDH'), which in turn had been generated from leucine and phenylalanine DH, respectively. Unlike the parent proteins, the chimeric AmDH ('cFL-AmDH') catalyzes the amination of acetophenone to (R)-methylbenzylamine and adamantylmethylketoneto adamantylethylamine.

引用

页码：14953 / 14955

页数：3

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