3D micromixer for nanoliposome synthesis: a promising advance in high mass productivity

被引:26
作者
Firmino, Priscilla C. O. S. [1 ]
Vianna, Savio S. V. [2 ]
da Costa, Ohanna M. M. M. [3 ]
Malfatti-Gasperini, Antonio A. [3 ]
Gobbi, Angelo L. [4 ]
Lima, Renato S. [4 ,5 ,6 ,7 ]
de la Torre, Lucimara G. [1 ]
机构
[1] Univ Campinas UNICAMP, Sch Chem Engn, Dept Mat & Bioproc Engn, Campinas, SP, Brazil
[2] Univ Campinas UNICAMP, Sch Chem Engn, Dept Chem Syst Engn, Campinas, SP, Brazil
[3] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Synchrotron Light Lab LNLS, BR-13083970 Campinas, SP, Brazil
[4] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Nanotechnol Natl Lab LNNano, BR-13083970 Campinas, SP, Brazil
[5] Univ Campinas UNICAMP, Inst Chem, BR-13083970 Campinas, SP, Brazil
[6] Univ Sao Paulo, Sao Carlos Inst Chem, BR-09210580 Sao Carlos, SP, Brazil
[7] Fed Univ ABC, BR-09210580 Sao Paulo, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
CONTINUOUS-FLOW PRODUCTION; MICROFLUIDIC DEVICE; CATIONIC LIPOSOMES; ETHANOL; DIFFUSION; MICROCHANNELS; NANOPARTICLES; TURBULENCE; DELIVERY; DESIGN;
D O I
10.1039/d1lc00232e
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This paper addresses an important breakthrough in the high mass production of liposomes by microfluidics technology. We investigated the synthesis of liposomes using a high flow rate microfluidic device (HFR-MD) with a 3D-twisted cross-sectional microchannel to favor chaotic advection. A simple construction scaffold technique was used to manufacture the HFR-MD. The synthesis of liposomes combined the effects of high flow and high concentration of lipids, resulting in high mass productivity (2.27 g of lipid per h) which, to our knowledge, has never been registered by only one microdevice. We assessed the effects of the flow rate ratio (FRR), total flow rate (TFR), and lipid concentration on the liposome physicochemical properties. HFR-MD liposomes were monodisperse (0.074) with a size around 100 nm under the condition of an FRR of 1 (50% v/v ethanol) and TFR of 5 ml min(-1) (expandable to 10 ml min(-1)). We demonstrated that the mixing conditions are not the only parameter controlling liposome synthesis using experimental and computational fluid dynamics analysis. A vacuum concentrator was used for ethanol removal, and there is no further modification after processing in accordance with the structural (SAXS) and morphological (cryo-TEM) analysis. Hence, the HFR-MD can be used to prepare nanoliposomes. It emerges as an innovative tool with high mass production.
引用
收藏
页码:2971 / 2985
页数:15
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