Conjugated linoleic acids can change phagocytosis of human Monocytes/Macrophages by reduction in Cox-2 expression

被引:35
作者
Stachowska, Ewa [1 ]
Baskiewicz-Masiuk, Magdalena
Dziedziejko, Violetta
Adler, Grazyna
Bober, Joanna
Machalinski, Boguslaw
Chlubek, Dariusz
机构
[1] Pomeranian Med Univ, Dept Biochem, Szczecin, Poland
[2] Pomeranian Med Univ, Dept Gen Pathol, Szczecin, Poland
[3] Pomeranian Med Univ, Dept Clin Biochem & Lab Diagnost, Szczecin, Poland
[4] Pomeranian Med Univ, Dept Med Chem, Szczecin, Poland
关键词
conjugated linoleic acid; monocytes/macrophages; phagocytosis; NF-kappa B; prostaglandin E2; NF-KAPPA-B; ACTIVATED RECEPTOR-GAMMA; LIPOPOLYSACCHARIDE-INDUCED CYCLOOXYGENASE; POLYUNSATURATED FATTY-ACIDS; ENDOTHELIAL-CELLS; PHORBOL ESTER; PPAR-GAMMA; MACROPHAGES; INFLAMMATION; INHIBITION;
D O I
10.1007/s11745-007-3072-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Prostaglandin E-2 produced endogenously (by cyclooxygenases) can regulate macrophage phagocytosis. Cyclooxygenase activity reduction (mainly through inhibition of inducible Cox-2) can induce PGE(2) synthesis depression and can activate the phagocytosis process. There are no reports in the literature explaining whether conjugated linoleic acid dienes (trans-10, cis-12 CLA and cis-9, trans-11 CLA) modify the phagocytic activity of human macrophages. For the purpose of this study, monocytes were isolated from venous blood, incubated for 7 days with 30 mu M CLAs, and then (in some experiments) LPS (1 mu g/mL) was added to the medium. Subsequently, monocyte/macrophage phagocytosis, NF-kappa B transcription factor activity, Cox-2 and PPAR gamma mRNA expression (and the amounts of Cox-2 and PPAR gamma proteins) and PGE(2) synthesis were determined. Both CLA isomers increased macrophage phagocytosis through inhibition of Cox-2 expression (might by inactivation the NF-kappa B pathway). The inhibition of mRNA Cox-2 expression contributed (particularly with respect to trans-10, cis-12 CLA) to a decrease in protein Cox-2 synthesis and to reduction of prostaglandin E-2 content in the cell. The inhibition of PGE(2) synthesis (by CLA treatment) enhanced the phagocytosis process in macrophages.
引用
收藏
页码:707 / 716
页数:10
相关论文
共 59 条
[1]   Prostaglandin E2 inhibits alveolar macrophage phagocytosis through an E-prostanoid 2 receptor-mediated increase in intracellular cyclic AMP [J].
Aronoff, DM ;
Canetti, C ;
Peters-Golden, M .
JOURNAL OF IMMUNOLOGY, 2004, 173 (01) :559-565
[2]   Conjugated linoleic acids (CLA) as precursors of a distinct family of PUFA [J].
Banni, S ;
Petroni, A ;
Blasevich, M ;
Carta, G ;
Cordeddu, L ;
Murru, E ;
Melis, MP ;
Mahon, A ;
Belury, MA .
LIPIDS, 2004, 39 (11) :1143-1146
[3]   Conjugated linoleic acid is an activator and ligand for peroxisome proliferator-activated receptor-gamma (PPARγ) [J].
Belury, MA ;
Moya-Camarena, SY ;
Lu, M ;
Shi, LL ;
Leesnitzer, LM ;
Blanchard, SG .
NUTRITION RESEARCH, 2002, 22 (07) :817-824
[4]   Nuclear factor-κB, cancer, and apoptosis [J].
Bours, V ;
Bentires-Alj, M ;
Hellin, AC ;
Viatour, P ;
Robe, P ;
Delhalle, S ;
Benoit, V ;
Merville, MP .
BIOCHEMICAL PHARMACOLOGY, 2000, 60 (08) :1085-1089
[5]   Incorporation of cis-9,trans-11 or trans-10,cis-12 conjugated linoleic acid into plasma and cellular lipids in healthy men [J].
Burdge, GC ;
Lupoli, B ;
Russell, JJ ;
Tricon, S ;
Kew, S ;
Banerjee, T ;
Shingfield, KJ ;
Beever, DE ;
Grimble, RF ;
Williams, CM ;
Yaqoob, P ;
Calder, PC .
JOURNAL OF LIPID RESEARCH, 2004, 45 (04) :736-741
[6]   Constitutive nuclear factor-κB activity preserves homeostasis of quiescent mature lymphocytes and granulocytes by controlling the expression of distinct Bcl-2 family proteins [J].
Bureau, F ;
Vanderplasschen, A ;
Jaspar, F ;
Minner, F ;
Pastoret, PP ;
Merville, MP ;
Bours, V ;
Lekeux, P .
BLOOD, 2002, 99 (10) :3683-3691
[7]   n-3 polyunsaturated fatty acids, inflammation, and inflammatory diseases [J].
Calder, Philip C. .
AMERICAN JOURNAL OF CLINICAL NUTRITION, 2006, 83 (06) :1505S-1519S
[8]   Contribution of conjugated linoleic acid to the suppression of inflammatory responses through the regulation of the NF-κB pathway [J].
Cheng, WL ;
Lii, CK ;
Chen, HW ;
Lin, TH ;
Liu, KL .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (01) :71-78
[9]   NF-κB:: pivotal mediator or innocent bystander in atherogenesis? [J].
Collins, T ;
Cybulsky, MI .
JOURNAL OF CLINICAL INVESTIGATION, 2001, 107 (03) :255-264
[10]   The inhibition of endothelial activation by unsaturated fatty acids [J].
De Caterina, R ;
Spiecker, M ;
Solaini, G ;
Basta, G ;
Bosetti, F ;
Libby, P ;
Liao, J .
LIPIDS, 1999, 34 :S191-S194