Human gene targeting by viral vectors

被引：232

作者：

Russell, DW ^{[1
]}

Hirata, RK

机构：

[1] Univ Washington, Sch Med, Markey Mol Med Ctr, Seattle, WA 98195 USA

[2] Univ Washington, Sch Med, Dept Med, Div Hematol, Seattle, WA 98195 USA

来源：

NATURE GENETICS | 1998年 / 18卷 / 04期

关键词：

D O I：

10.1038/ng0498-325

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Stable transduction of mammalian cells typically involves random integration of viral vectors by non-homologous recombination. Here we report that Vectors based on adeno-associated virus (AAV) can efficiently modify homologous human chromosomal target sequences. Bath integrated neomycin phosphotransferase genes and the hypoxanthine phosphoribosyltransferase gene were targeted by AAV vectors. Site-specific genetic modifications could be introduced into approximately 1% of cells, with the highest targeting rates occurring in normal human fibroblasts. These results suggest that AAV vectors could be used to introduce specific genetic changes into the genomic DNA of a wide variety of mammalian cells. including therapeutic gene targeting applications.

引用

页码：325 / 330

页数：6

共 36 条

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