Evaluation of Neuraminidase Antigen Based Competitive Enzyme-Linked Immunosorbent Assay in Chickens Vaccinated with Avian Influenza Inactivated Vaccine

被引：4

作者：

Kim, Jong-Nyeo ^{[1
]}

Byun, Seong-Hwan ^{[1
]}

Kang, Shien-Young ^{[1
]}

Mo, In-Pil ^{[1
]}

机构：

[1] Chungbuk Natl Univ, Coll Vet Med, Avian Dis Lab, Cheongju 361763, South Korea

来源：

AVIAN DISEASES | 2010年 / 54卷 / 01期

关键词：

avian influenza; ELISA; vaccination; neuraminidase; VIRUS; VALIDATION; DIAGNOSIS;

D O I：

10.1637/8747-033009-ResNote.1

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A competitive enzyme-linked immunosorbent assay (c-ELISA) was developed as a serologic diagnostic tool to detect antibodies against NA subtype 3 of avian influenza virus (AIV). The NA antigen used in this c-ELISA was obtained by pronase treatment of allantoic fluid of specific-pathogen-free (SPF) eggs infected with AIV. The NA specific monoclonal antibodies were produced from purified NA. The N3 c-ELISA was carried out on scrum samples collected from both SPF chickens and commercial layers to confirm whether the N3 c-ELISA was capable of detecting specific N3 antibodies. The positive cutoff percentage inhibition value was 6.13%. The sensitivity and specificity of the N3 c-ELISA were 83.7% and 95.6%, respectively, which indicated that N3 c-ELISA can detect the antibodies from SPF chickens or commercial chickens vaccinated with H9N3 subtype of AIV.

引用

页码：682 / 685

页数：4

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