Globular adiponectin augments from pancreatic islet β cells at insulin secretion high glucose concentrations

被引:46
作者
Gu, Weiqiong
Li, Xiaoying
Liu, Changqin
Yang, Jun
Ye, Lei
Tang, Jingfen
Gu, Yuanjun
Yang, Yisheng
Hong, Jie
Zhang, Yifei
Chen, Mingdao
Ning, Guang
机构
[1] Shanghai Jiao Tong Univ, Sch Med, Rui Jin Hosp,Dept Endocrinol & Metab, Shanghai Clin Ctr Endocrine & Metab Dis, Shanghai 200025, Peoples R China
[2] E Inst Shanghai Univ, Endocrine & Metab Div, Shanghai 200025, Peoples R China
关键词
adiponectin; islet beta cells; insulin secretion; AMPK;
D O I
10.1385/ENDO:30:2:217
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Adiponectin plays an important role in improving insulin resistance and preventing atherosclerosis. However it has been rarely reported that adiponectin influences insulin secretion because its receptor was identified in human islet P cells. In order to investigate the direct effect of adiponectin on pancreatic islet 0 cells, we performed an insulin secretion test in purified rat islets, which were incubated with adiponectin (100 ng/mL) at low (3.3 mA4) and high (16.7 mM) glucose concentrations. Furthermore, cell lysates were extracted from the adiponectin-treated islets for p-AMPK alpha assay. RTPCR and immunohistochemical examination showed both adiponectin receptor 1 (AdipoR1) and receptor 2 (AdipoR2) were expressed in islet cells and AdipoR1 was predominantly expressed. Insulin secretion was significantly increased in the presence of adiponectin for 6 h at high glucose concentration. Meanwhile, the levels of phosphorylated AMPK increased with adiponectin treatment at high glucose concentrations. It is concluded that adiponectin augments insulin secretion from pancreatic islet P cells at high glucose concentration through AMPK activation.
引用
收藏
页码:217 / 221
页数:5
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