Polysaccharides from by-products of the Wonderful and Laffan pomegranate varieties: New insight into extraction and characterization

被引:40
作者
Khatib, Mohamad [1 ]
Giuliani, Camilla [1 ]
Rossi, Federico [3 ]
Adessi, Alessandra [3 ]
Al-Tamimi, Amal [2 ]
Mazzola, Giuseppe [4 ]
Di Gioia, Diana [4 ]
Innocenti, Marzia [1 ]
Mulinacci, Nadia [1 ]
机构
[1] Univ Florence, Pharmaceut & Nutraceut Sect, Dept Neurosci Psychol Drug & Child Hlth, Via Ugo Schiff 6, Sesto Fiorentino, Fl, Italy
[2] Princess Noura Univ, Biol Dept Ecol, Al Imam Abdullah Ibn Saud Ibn Abdul Aziz Rd, Riyadh, Saudi Arabia
[3] Univ Florence, Dept Sci Agrifood Prod & Environm, Piazzale Cascine 18, I-50144 Florence, Italy
[4] Univ Bologna, Dept Agr Sci, Viale Fanin 44, I-40127 Bologna, Italy
关键词
Mesocarp; Prebiotic activity; Pectin; Size exclusion chromatography; H-1; NMR; GUT MICROBIOTA; PEEL; OPTIMIZATION; ANTIOXIDANT; FRUIT; RIND;
D O I
10.1016/j.foodchem.2017.05.041
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The main crude polysaccharides (CPS), extracted from two widely cultivated pomegranate varieties, Laffan and Wonderful, were studied and characterized. We obtained the highest CPS extraction yield (approximatively 10% wow on dried matter) by 1 h of decoction (ratio 1/40 w/v). The predominant polymers (75-80%) of the CPS samples showed a hydrodynamic volume close to 2000 kDa by size exclusion chromatography and the exocarp and mesocarp profiles were very similar. The proton spectra (H-1 NMR), according to sugar composition and gelling ability, confirmed the main polysaccharide fractions were pectin with different acylation and methylation degree. The CPS from Laffan and Wonderful mesocarp showed prebiotic properties in vitro with Lactobacillus and Bifidobacterium strains. The composition of the decoction (12% ellagitannins and 10% of CPS) obtained by a green extraction process of pomegranate by-products, makes it a suitable component of functional food formulations. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码:58 / 66
页数:9
相关论文
共 39 条
[1]   Pomegranate peel and peel extracts: Chemistry and food features [J].
Akhtar, Saeed ;
Ismail, Tariq ;
Fraternale, Daniele ;
Sestili, Piero .
FOOD CHEMISTRY, 2015, 174 :417-425
[2]   Characterization of Bifidobacterium spp. strains for the treatment of enteric disorders in newborns [J].
Aloisio, Irene ;
Santini, Cecilia ;
Biavati, Bruno ;
Dinelli, Giovanni ;
Cencic, Avrelija ;
Chingwaru, Walter ;
Mogna, Luca ;
Di Gioia, Diana .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2012, 96 (06) :1561-1576
[3]  
[Anonymous], 2005, FOOD CARBOHYDRATES
[4]  
[Anonymous], 012014 EUR, P1
[5]   Rapid quantification of O-acetyl and O-methyl residues in pectin extracts [J].
Bédouet, L ;
Courtois, B ;
Courtois, J .
CARBOHYDRATE RESEARCH, 2003, 338 (04) :379-383
[6]   Macromolecular and chemical features of the excreted extracellular polysaccharides in induced biological soil crusts of different ages [J].
Chen, Lanzhou ;
Rossi, Federico ;
Deng, Songqiang ;
Liu, Yongding ;
Wang, Gaohong ;
Adessi, Alessandra ;
De Philippis, Roberto .
SOIL BIOLOGY & BIOCHEMISTRY, 2014, 78 :1-9
[7]   Differentiation of the characteristics of excreted extracellular polysaccharides reveals the heterogeneous primary succession of induced biological soil crusts [J].
Colica, Giovanni ;
Li, Hua ;
Rossi, Federico ;
De Philippis, Roberto ;
Liu, Yongding .
JOURNAL OF APPLIED PHYCOLOGY, 2015, 27 (05) :1935-1944
[8]   Flavonoid bioconversion in Bifidobacterium pseudocatenulatum B7003: A potential probiotic strain for functional food development [J].
Di Gioia, Diana ;
Strahsburger, Erwin ;
Lopez de Lacey, Ana M. ;
Bregola, Valeria ;
Marotti, Ilaria ;
Aloisio, Irene ;
Biavati, Bruno ;
Dinelli, Giovanni .
JOURNAL OF FUNCTIONAL FOODS, 2014, 7 :671-679
[9]   Bifidobacteria: their impact on gut microbiota composition and their applications as probiotics in infants [J].
Di Gioia, Diana ;
Aloisio, Irene ;
Mazzola, Giuseppe ;
Biavati, Bruno .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2014, 98 (02) :563-577
[10]   STRUCTURE OF THE CAPSULAR POLYSACCHARIDE FROM THE KLEBSIELLA K8 REFERENCE STRAIN-1015 [J].
ERBING, B ;
JANSSON, PE ;
WIDMALM, G ;
NIMMICH, W .
CARBOHYDRATE RESEARCH, 1995, 273 (02) :197-205