Microsatellite DNA analysis proves nucleus of interspecies reconstructed blastocyst coming from that of donor giant panda

被引：3
作者：
Ding, B
Shi, P
Xiangyu, JG
Zhang, YP [1 ]
Chen, DY
Sun, QY
Li, GP
Wang, MK
Liu, YL
Kang, L
Han, ZM
Song, XF
Li, JS
Chen, YC
机构：
[1] Chinese Acad Sci, Kunming Inst Zool, Lab Cellular & Mol Evolut, Kunming 650223, Peoples R China
[2] Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100080, Peoples R China
[3] Fuzhou Panda Inst Ctr, Fuzhou 350001, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 2000年 / 45卷 / 20期
关键词：
giant panda (Ailuropoda melanoleuca); interspecies cloning; microsatellite DNA; nucleus;
D O I：
10.1007/BF02886299
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A method for DNA isolation from early development of blastocyst and further analysis of nuclear and mitochondrial DNA was developed in present study. Total DNA was prepared from interspecies reconstructed blastocyst and a giant panda specific microsatellite locus g(010) was successfully amplified. DNA sequencing of the PCR product showed that two sequences of reconstructed blastocysts are the same as that of positive control giant panda. Our results prove that the nucleus of interspecies reconstructed blastocyst comes from somatic nucleus of donor giant panda.
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收藏
页码：1883 / 1885
页数：3
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