Development of an indirect enzyme-linked immunosorbent assay for the detection of osmotolerant yeast Zygosaccharomyces rouxii in different food

被引：6
作者：
Guo, Hong [1 ,2 ,3 ]
Yuan, Ya-Hong [1 ,2 ,3 ]
Niu, Chen [1 ,2 ,3 ]
Qiu, Yue [1 ,2 ,3 ]
Wei, JianPing [1 ,2 ,3 ]
Yue, Tian-Li [1 ,2 ,3 ]
机构：
[1] Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China
[2] Minist Agr, Lab Qual & Safety Risk Assessment Agro Prod Yangl, Yangling 712100, Shaanxi, Peoples R China
[3] Natl Engn Res Ctr Agr Integrat Test Yangling, Yangling 712100, Shaanxi, Peoples R China
来源：
FOOD AND AGRICULTURAL IMMUNOLOGY | 2018年 / 29卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Zygosaccharomyces rouxii; polyclonal antibodies; indirect ELISA; immuno-detection; food quality; APPLE JUICE CONCENTRATE; ALICYCLOBACILLUS-ACIDOTERRESTRIS; RAPID IDENTIFICATION; ELECTRONIC NOSE; SACCHAROMYCES-CEREVISIAE; SPOILAGE; ANTIBODIES; SUGAR; IMMUNOASSAY; STRAINS;
D O I：
10.1080/09540105.2018.1491532
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A polyclonal antibody-based indirect ELISA for the sensitive and specific detection of osmotolerant Zygosaccharomyces rouxii was established. The reactivity of the polyclonal antibodies (pAbs) with Z.rouxii was confirmed by immunofluorescence. The ELISA detection limit was 5 x 10(4) CFU/mL. For an evaluation of the specificity of pAbs, reactivity to 10 fungi species and 2 bacteria species commonly found in food environments was analysed by indirect ELISA. ELISA reacts with all tested Z.rouxii, whereas no cross-reactions or weakly reactions with 9 fungi and 2 bacteria were observed. The immunoassay staining confirmed the ELISA. Further on, the assay applicability was tested with concentrated apple juice, wine, and honey. Food samples were inoculated with 100 CFU/mL of Z.rouxii. After enrichment for 24h, the established ELISA can successfully detect contaminated wine and concentrated apple juice samples, while only 30h was required for honey samples.
引用
收藏
页码：976 / 988
页数：13
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