A Covalent Succinylcysteine-like Intermediate in the Enzyme-Catalyzed Transformation of Maleate to Fumarate by Maleate Isomerase

被引：33

作者：

Fisch, Florian ^{[1
]}

Fleites, Carlos Martinez ^{[1
]}

Delenne, Marie ^{[1
]}

Baudendistel, Nina ^{[3
]}

Hauer, Bernhard ^{[3
]}

Turkenburg, Johan P. ^{[1
]}

Hart, Sam ^{[1
]}

Bruce, Neil C. ^{[2
]}

Grogan, Gideon ^{[1
]}

机构：

[1] Univ York, York Struct Biol Lab, Dept Chem, York YO10 5YW, N Yorkshire, England

[2] Univ York, Ctr Novel Agr Prod, Dept Biol, York YO10 5YW, N Yorkshire, England

[3] GVF E, BASF SE, Ludwigshafen, Germany

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 33期

关键词：

CIS-TRANS ISOMERASE; SUBTILIS GLUTAMATE RACEMASE; ARYLMALONATE DECARBOXYLASE; CRYSTAL-STRUCTURE; ESCHERICHIA-COLI; MECHANISM; EVOLUTION; CLONING; ACID;

D O I：

10.1021/ja1053576

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Maleate isomerase (MI), a member of the Asp/Glu racemase superfamily, catalyzes cis-trans isomerization of the C2-C3 double bond in maleate to yield fumarate. Mutational studies, in conjunction with the structure of the C194A mutant of Nocardia farcinica MI cocrystallized with maleate, have revealed an unprecedented mode of catalysis for the superfamily in which the isomerization reaction is initiated by nucleophilic attack of cysteine at the double bond, yielding a covalent succinylcysteine-like intermediate.

引用

页码：11455 / 11457

页数：3

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