Manure contaminated with the antibiotic sulfadiazine impairs the abundance of nirK- and nirS-type denitrifiers in the gut of the earthworm Eisenia fetida

被引：14

作者：

Kotzerke, Anja ^{[1
]}

Klemer, Sven ^{[1
]}

Kleineidam, Kristina ^{[2
]}

Horn, Marcus A. ^{[3
]}

Drake, Harold L. ^{[3
]}

Schloter, Michael ^{[2
]}

Wilke, Berndt-Michael ^{[1
]}

机构：

[1] Berlin Univ Technol, Inst Ecol, D-10587 Berlin, Germany

[2] Helmholtz Zentrum Munchen, Inst Soil Ecol, D-85764 Neuherberg, Germany

[3] Univ Bayreuth, Dept Ecol Microbiol, D-95440 Bayreuth, Germany

来源：

BIOLOGY AND FERTILITY OF SOILS | 2010年 / 46卷 / 04期

关键词：

Denitrifiers; nirK; nirS; Real-time PCR; Antibiotic; Sulfadiazine; Worm gut; Eisenia fetida; IN-VIVO EMISSION; REDUCTASE GENES NIRK; NITROUS-OXIDE N2O; N2O-PRODUCING MICROORGANISMS; LUMBRICUS-RUBELLUS; PIG-MANURE; BACTERIA; SOIL; COMMUNITY; HABITAT;

D O I：

10.1007/s00374-009-0434-3

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

The antibiotic sulfadiazine (SDZ) can affect denitrifying bacteria in soil. However, effects on denitrifiers in the gut of earthworms have not been described so far. Therefore, the influence of SDZ-contaminated manure applied to soil on denitrifiers in the gut of the earthworm Eisenia fetida was assessed by quantitative polymerase chain reaction targeting genes coding for nirK- and nirS-type nitrite reductases of denitrifiers. Gut contents of Eisenia fetida contained 2.5 x 10(6) and 5.1 x 10(5) gene copies of nirK and nirS, respectively, after 2 weeks in soils amended with manure only. Copy numbers of nirK and nirS in gut contents from manure treatments with SDZ were up to ten times less. Overall, the data indicate a negative impact of SDZ on denitrifiers in the gut of earthworms.

引用

页码：415 / 418

页数：4

共 2 条

[1] Manure contaminated with the antibiotic sulfadiazine impairs the abundance of nirK- and nirS-type denitrifiers in the gut of the earthworm Eisenia fetida
Anja Kotzerke
Sven Klemer
Kristina Kleineidam
Marcus A. Horn
Harold L. Drake
Michael Schloter
Berndt-Michael Wilke
Biology and Fertility of Soils, 2010, 46 : 415 - 418
[2] Distinct distribution patterns of proteobacterial nirK- and nirS-type denitrifiers in the Yellow River estuary, China
Li, Fenge
Li, Mingcong
Shi, Wenchong
Li, Han
Sun, Zhongtao
Gao, Zheng
CANADIAN JOURNAL OF MICROBIOLOGY, 2017, 63 (08) : 708 - 718

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