Rind-pith separation of corn stalks is one of the effective measures for realizing high-value utilization of stalks. Therefore, it is necessary to study rind-pith adhesive strength of stalks and its influencing factors to determine effective separation methods. In this study, a peeling method was put forward and single factor and multi factor tests were carried out to explore the rind-pith adhesive strength. The analysis of single factor tests showed that sampling location, moisture content, and peeling speed were all significant on the average rind-pith peeling strength and the F-p = 25.70 > F-0(.)05 (4, 20) = 2.87, F-m = 112.46 > F-0.(05) (4, 20) = 2.87 and F-s = 231.21 > F-0(.05) (6, 28) = 2.45. The mathematical model between average peeling strength and influencing factors was established through multi factor tests. The model was analyzed and optimized, and the significance sequence of influencing factors is sampling location > moisture content > peeling speed, and the effect of interaction between moisture content and sampling location was significant (P<0.05). It can be concluded that the rind-pith peeling strengths of internode sections among 1st-7th internodes of corn stalks were 360.09-450.01 N/m, which are smaller and more favorable for mechanical separation for stalks with moisture content of 15%-20% and at peeling speed of 30-40 mm/min. The results of this study may provide a theoretical reference for the modeling of corn stalks and the subsequent simulation and optimization of rind-pith separation machines.