Upregulation of purinergic P2Y receptor-mediated calcium responses in glial cells during experimental detachment of the rabbit retina

被引:11
作者
Uckermann, O
Uhlmann, S
Weick, M
Pannicke, T
Francke, M
Reichenbach, A
Wiedemann, P
Bringmann, A
机构
[1] Univ Leipzig, Fac Med, Dept Ophthalmol, Eye Clin, D-04103 Leipzig, Germany
[2] Paul Flechsig Inst Brain Res, Dept Neurophysiol, D-04109 Leipzig, Germany
关键词
adenosine 5 '-triphosphate; P2; receptor; Muller glial cell; gliosis; retinal detachment; retina;
D O I
10.1016/S0304-3940(02)01402-7
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
To investigate injury-induced alterations of purinergic P2Y receptor-mediated calcium responses in glial (Muller) cells of the rabbit retina, neural retinae were experimentally detached from the pigment epithelium. The ATP-evoked calcium responses were recorded in the endfeet of glial cells at the vitread surface of retinal wholemounts. In control retinae, approximately 7% of the glial cells investigated showed ATP-evoked calcium responses. Within 24 h of detachment, significantly more retinal glial cells (42%) showed calcium responses, and glial ATP responsiveness increased further in retinae which were detached for 48 (44%) or for 72 h (64%). The results indicate that in the detached retina, glial cells upregulate their responsiveness to extracellular ATP within 24 h of injury. Thus, P2Y receptor-mediated signalling may be involved in the early steps of glial response to retinal injury. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:131 / 134
页数:4
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