EpCAMlow Circulating Tumor Cells: Gold in the Waste

被引:25
作者
Nicolazzo, Chiara [1 ]
Gradilone, Angela [1 ]
Loreni, Flavia [1 ]
Raimondi, Cristina [2 ]
Gazzaniga, Paola [1 ]
机构
[1] Sapienza Univ Rome, Dept Mol Med, I-00161 Rome, Italy
[2] Sapienza Univ Rome, Dept Radiol Oncol & Anatomopathol Sci, I-00161 Rome, Italy
关键词
EPITHELIAL-MESENCHYMAL TRANSITION; CANCER; METASTASIS; BEVACIZUMAB; PROGRESSION; CORRELATE; PROSTATE;
D O I
10.1155/2019/1718920
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The CellSearch (R) system which is still considered the gold standard for the enumeration of circulating tumor cells (CTC) utilizes antibodies against the epithelial cell adhesion molecule (EpCAM) for CTC enrichment. Recently, CTC discarded by the CellSearch (R) system due to their low EpCAM expression have been isolated and analyzed. We here sought to discuss technical and biological issues concerning the isolation and characterization of EpCAM(low) CTC, highlighting the enormous potential of this subpopulation discarded by CellSearch (R), which might instead reveal an unexpected clinical significance in tumor types where CTC enumeration has never been validated for prognostic and predictive purpose.
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页数:5
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