Utilization of desugarized sugar beet molasses for the production of poly(3-hydroxybutyrate) by halophilic Bacillus megaterium uyuni S29

被引：30

作者：

Schmid, Maximilian T. ^{[1
]}

Song, Hyunjeong ^{[1
]}

Raschbauer, Michaela ^{[1
]}

Emerstorfer, Florian ^{[2
]}

Omann, Markus ^{[2
]}

Stelzer, Franz ^{[3
]}

Neureiter, Markus ^{[1
]}

机构：

[1] Univ Nat Resources & Life Sci, Inst Environm Biotechnol, Tulln, Austria

[2] AGRANA Res & Innovat Ctr GmbH, Tulln, Austria

[3] Univ Technol, Inst Chem & Technol Mat, Graz, Austria

来源：

PROCESS BIOCHEMISTRY | 2019年 / 86卷

关键词：

Bacillus megaterium; Desugarized sugar beet molasses; Poly(3-hydroxybutyrate); Halophilic fermentation; Sugar industry by-product; PHA PRODUCTION; POLYHYDROXYALKANOATES; BIOMASS;

D O I：

10.1016/j.procbio.2019.08.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Desugarized sugar beet molasses is a high-salinity side stream after fractionation of beet molasses by chromatography. It could be demonstrated that this by-product of the sugar industry is a suitable substrate for the production of poly(3-hydroxybutyrate) with the halophilic bacterium Bacillus megaterium uyuni S29. Cell dry mass concentrations of up to 16.7 g L-1 with a P(3HB) content of 0.6 g g(-1) were achieved after only 24 h of batch cultivation. Utilization of a desugarized sugar beet molasses medium resulted in a two- to threefold increase in biomass production compared to a conventional mineral based medium. Depending on the extraction method, the molecular mass (M-n) of the P(3HB) varied between 119 kDa and 722 kDa. The high P(3HB) productivity of 0.42 g L-1 h(-1) during batch experiments combined with desugarized sugar beet molasses as inexpensive substrate suggests a simple and robust process for cost effective P(3HB) production.

引用

页码：9 / 15

页数：7