A bienzymatic amperometric glucose biosensor based on using a novel recombinant Mn peroxidase from corn and glucose oxidase with a Nafion membrane

Using a recombinant enzyme derived from corn and a simple modification, we fabricated a facile, fast, and cost-beneficial novel biosensor to measure glucose. The Nafion/Plant Produced Mn Peroxidase (PPMP)-glucose oxidase (GOx)-Bovine serum albumin (BSA)/Au electrode showed an excellent amperometric response to detect glucose. This biosensor is capable of responding to a wide range of glucose, 20.0 mu M-15.0 mM, and has a lower detection limit (LOD) of 2.9 mu M. The reproducibility response using six electrodes is also very substantial and indicates high capability of this biosensor to detect glucose concentrations. Selectivity of this electrode was investigated in an optimized experimental solution containing 10% diet green tea with citrus containing ascorbic acid (AA), and citric acid (CA) in a wide concentration of glucose at 0.02-14.0 mM with an LOD of 3.1 mu M. We also applied linear sweep voltammetry (LSV) and this technique shows a wide range of glucose detection. The performance and strength of this novel enzyme biosensor were the simplicity, wide linear ranges, sensitivities, selectivity, and low limits of detection. We expect that the modified biosensor has the potential for monitoring various biofluids.