A Global Protein Kinase and Phosphatase Interaction Network in Yeast

被引：515

作者：

Breitkreutz, Ashton ^{[1
]}

Choi, Hyungwon ^{[2
]}

Sharom, Jeffrey R. ^{[1
,3
]}

Boucher, Lorrie ^{[1
]}

Neduva, Victor ^{[4
,5
]}

Larsen, Brett ^{[1
]}

Lin, Zhen-Yuan ^{[1
]}

Breitkreutz, Bobby-Joe ^{[1
]}

Stark, Chris ^{[1
]}

Liu, Guomin ^{[1
]}

Ahn, Jessica ^{[1
]}

Dewar-Darch, Danielle ^{[1
]}

Reguly, Teresa ^{[1
]}

Tang, Xiaojing ^{[1
]}

Almeida, Ricardo ^{[4
,5
]}

Qin, Zhaohui Steve ^{[6
]}

Pawson, Tony ^{[1
,3
]}

Gingras, Anne-Claude ^{[1
,3
]}

Nesvizhskii, Alexey I. ^{[2
,7
]}

Tyers, Mike ^{[1
,3
,4
,5
]}

机构：

[1] Samuel Lunenfeld Res Inst, Ctr Syst Biol, Toronto, ON M5G 1X5, Canada

[2] Univ Michigan, Dept Pathol, Ann Arbor, MI 48109 USA

[3] Univ Toronto, Dept Mol Genet, Toronto, ON M5S 1A8, Canada

[4] Univ Edinburgh, Sch Biol Sci, Edinburgh EH9 3JR, Midlothian, Scotland

[5] Univ Edinburgh, Wellcome Trust Ctr Cell Biol, Edinburgh EH9 3JR, Midlothian, Scotland

[6] Univ Michigan, Dept Biostat, Ann Arbor, MI 48109 USA

[7] Univ Michigan, Ctr Computat Med & Bioinformat, Ann Arbor, MI 48109 USA

来源：

SCIENCE | 2010年 / 328卷 / 5981期

基金：

加拿大健康研究院; 美国国家卫生研究院;

关键词：

SACCHAROMYCES-CEREVISIAE; CELL-CYCLE; PHOSPHORYLATION; TRANSDUCTION; PROTEOMICS; FEEDBACK; MITOSIS; SIGNAL;

D O I：

10.1126/science.1176495

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The interactions of protein kinases and phosphatases with their regulatory subunits and substrates underpin cellular regulation. We identified a kinase and phosphatase interaction (KPI) network of 1844 interactions in budding yeast by mass spectrometric analysis of protein complexes. The KPI network contained many dense local regions of interactions that suggested new functions. Notably, the cell cycle phosphatase Cdc14 associated with multiple kinases that revealed roles for Cdc14 in mitogen-activated protein kinase signaling, the DNA damage response, and metabolism, whereas interactions of the target of rapamycin complex 1 (TORC1) uncovered new effector kinases in nitrogen and carbon metabolism. An extensive backbone of kinase-kinase interactions cross-connects the proteome and may serve to coordinate diverse cellular responses.

引用

页码：1043 / 1046

页数：4

共 22 条

[1] Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry [J].

Chi, An ;

Huttenhower, Curtis ;

Geer, Lewis Y. ;

Coon, Joshua J. ;

Syka, John E. P. ;

Bai, Dina L. ;

Shabanowitz, Jeffrey ;

Burke, Daniel J. ;

Troyanskaya, Olga G. ;

Hunt, Donald F. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (07) :2193-2198

[2] Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle [J].

Daub, Henrik ;

Olsen, Jesper V. ;

Bairlein, Michaela ;

Gnad, Florian ;

Oppermann, Felix S. ;

Koerner, Roman ;

Greff, Zoltan ;

Keri, Gyoergy ;

Stemmann, Olaf ;

Mann, Matthias .

MOLECULAR CELL, 2008, 31 (03) :438-448

[3] Self-perpetuating states in signal transduction: positive feedback, double-negative feedback and bistability [J].

Ferrell, JE .

CURRENT OPINION IN CELL BIOLOGY, 2002, 14 (02) :140-148

[4] Cell regulation: determined to signal discrete cooperation [J].

Gibson, Toby J. .

TRENDS IN BIOCHEMICAL SCIENCES, 2009, 34 (10) :471-482

[5] AMP-activated/SNF1 protein kinases: conserved guardians of cellular energy [J].

Hardie, D. Grahame .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2007, 8 (10) :774-785

[6] Characterization of the rapamycin-sensitive phosphoproteome reveals that Sch9 is a central coordinator of protein synthesis [J].

Huber, Alexandre ;

Bodenmiller, Bernd ;

Uotila, Aino ;

Stahl, Michael ;

Wanka, Stefanie ;

Gerrits, Bertran ;

Aebersold, Ruedi ;

Loewith, Robbie .

GENES & DEVELOPMENT, 2009, 23 (16) :1929-1943

[7] Tuning the Activation Threshold of a Kinase Network by Nested Feedback Loops [J].

Justman, Quincey A. ;

Serber, Zach ;

Ferrell, James E., Jr. ;

El-Samad, Hana ;

Shokat, Kevan M. .

SCIENCE, 2009, 324 (5926) :509-512

[8] The yeast cell-cycle network is robustly designed [J].

Li, FT ;

Long, T ;

Lu, Y ;

Ouyang, Q ;

Tang, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (14) :4781-4786

[9] Mitochondrial retrograde signaling [J].

Liu, Zhengchang ;

Butow, Ronald A. .

ANNUAL REVIEW OF GENETICS, 2006, 40 :159-185

[10] The transduction of the nitrogen regulation signal in Saccharomyces cerevisiae [J].

Magasanik, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (46) :16537-16538

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