Reduction of Oxidative Stress May Play a Role in the Anti-Inflammatory Effect of the Novel Herbal Formulation in a Rat Model of Hydrochloric Acid-Induced Cystitis

AimsWe investigated the effect of the multi-herbal medicine, WSY-1075 in an animal model of hydrochloric acid (HCl)-induced cystitis. MethodsRats were randomly assigned to three groups: sham-operated (control), HCl-induced only (HC), and HC treated with WSY-1075 (HC+WT). Oral administration of either distilled water (control, HC) or WSY-1075 (400mg/kg) was continued for 4 weeks. In HC and HC+WT groups, cystitis was induced with 0.4M HCl beginning on the 22nd day. Rats in each group underwent cystometrography, and bladders were examined for evidence of inflammation and oxidative stress. ResultsTreatment with WSY-1075 decreased the frequency of urination and reduced inflammation of the bladder tissue in a rat model of HCl-induced cystitis. Compared with the control group, the HC group showed severe chronic inflammatory and fibrosis signs, and the inflammatory grades significantly decreased following WSY-1075 treatment in the HC-WT group. The HC+WT group showed a markedly decreased expression of pro-inflammatory cytokines compared to the HC group. The level of malondialdehyde was significantly greater in the HC group compared to the control group, and it was significantly reduced in the treated (HC+WT) group. The levels of superoxide dismutase increased in the HC+WT group, which confirmed the anti-oxidant effect of WSY-1075. ConclusionsWe suggest that reduction of oxidative stress may play a role in this anti-inflammatory effect. Neurourol. Urodynam. 34:86-91, 2015. (c) 2013 Wiley Periodicals, Inc.