Comparison of real-time PCR methods for measurement of HIV-1 proviral DNA

被引：19
作者：
Rozera, G. [1 ]
Abbate, I. [1 ]
Bruselles, A. [1 ]
Bartolini, B. [1 ]
D'Offizi, G. [1 ]
Nicastri, E. [1 ]
Tommasi, C. [1 ]
Capobianchi, M. R. [1 ]
机构：
[1] INMI L Spallanzani, Rome, Italy
来源：
JOURNAL OF VIROLOGICAL METHODS | 2010年 / 164卷 / 1-2期
关键词：
Proviral burden; Real-time PCR; Marker; Sequence; ACTIVE ANTIRETROVIRAL THERAPY; CD4 CELL COUNT; DISEASE PROGRESSION; LOAD; RNA; INFECTION; QUANTIFICATION; QUANTITATION; LEVEL;
D O I：
10.1016/j.jviromet.2009.11.031
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The use of HIV-1 DNA quantitation in cellular reservoirs to predict disease progression and treatment outcome in infected patients is hampered by the lack of standardization among the available methods. In the present study, real-time PCR methods used commonly for HIV-1 proviral DNA evaluation were compared, showing strong differences in the results, probably as a consequence of genome variability in the target regions. Standardization of HIV-1 proviral DNA quantitation assays is needed for use in clinical management of patients with HIV-1. (C) 2010 Published by Elsevier B.V.
引用
收藏
页码：135 / 138
页数：4
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