Bacterial resistance modifying agents from Lycopus europaeus

被引:89
作者
Gibbons, S
Oluwatuyi, M
Veitch, NC
Gray, AI
机构
[1] Univ London, Sch Pharm, Ctr Pharmacognosy & Phytotherapy, London WC1N 1AX, England
[2] Royal Bot Gardens, Jodrell Lab, Richmond TW9 3DS, Surrey, England
[3] Univ Strathclyde, Dept Pharmaceut Sci, Glasgow G2 0NR, Lanark, Scotland
基金
英国工程与自然科学研究理事会;
关键词
Lamiaceae; Lycopus europaeus; Staphylococcus aureus; multidrug resistance; MDR; modulation; isopimarane diterpenes;
D O I
10.1016/S0031-9422(02)00446-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
As part of an ongoing project to identify plant natural products which modulate bacterial multidrug resistance (MDR), bioassay-guided isolation of an extract of Lycopus europaeus yielded two new isopimarane diterpenes, namely methyl-1alpha-acetoxy-7alpha 14alpha-dihydroxy-8,15-isopimaradien-18-oate (1) and methyl-1alpha,14alpha-diacetoxy-7alpha-hydroxy-8,15-isopimaradien-18-oate (2). The structures were established by spectroscopic methods. These compounds and several known diterpenes were tested for in vitro antibacterial and resistance modifying activity against strains of Staphylococcus aureus possessing the Tet(K), Msr(A), and Nor(A) multidrug resistance efflux mechanisms. At 512 mug/ml none of the compounds displayed any antibacterial activity but individually in combination with tetracycline and erythromycin, a two-fold potentiation of the activities of these antibiotics was observed against two strains of S. aureus that were highly resistant to these agents due to the presence of the multidrug efflux mechanisms Tet(K) (tetracycline resistance) and Msr(A) (macrolide resistance). (C) 2003 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:83 / 87
页数:5
相关论文
共 20 条
[1]   EFFECTS OF INDOLE ALKALOIDS ON MULTIDRUG RESISTANCE AND LABELING OF P-GLYCOPROTEIN BY A PHOTOAFFINITY ANALOG OF VINBLASTINE [J].
BECK, WT ;
CIRTAIN, MC ;
GLOVER, CJ ;
FELSTED, RL ;
SAFA, AR .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 153 (03) :959-966
[2]   NATURALLY-OCCURRING TERPENE DERIVATIVES .422. NEW SESQUITERPENE LACTONES, GERANYLLINALOL DERIVATIVES AND OTHER CONSTITUENTS FROM GEIGERIA SPECIES [J].
BOHLMANN, F ;
ZDERO, C ;
AHMED, M .
PHYTOCHEMISTRY, 1982, 21 (07) :1679-1691
[3]   FLAVONE GLUCURONIDES OF LYCOPUS-VIRGINICUS [J].
BUCAR, F ;
KARTNIG, T .
PLANTA MEDICA, 1995, 61 (04) :378-380
[4]  
Gibbons S, 2000, PHYTOTHER RES, V14, P139, DOI 10.1002/(SICI)1099-1573(200003)14:2&lt
[5]  
139::AID-PTR608&gt
[6]  
3.0.CO
[7]  
2-8
[8]   Infections due to vancomycin-resistant Enterococcus faecium resistant to linezolid [J].
Gonzales, RD ;
Schreckenberger, PC ;
Graham, MB ;
Kelkar, S ;
DenBesten, K ;
Quinn, JP .
LANCET, 2001, 357 (9263) :1179-1179
[9]   Diterpenoids from Lycopus europaeus and Nepeta septemcrenata:: Revised structures and new isopimarane derivatives [J].
Hussein, AA ;
Rodríguez, B ;
Martínez-Alcázar, MD ;
Cano, FH .
TETRAHEDRON, 1999, 55 (23) :7375-7388
[10]   Isopimarane diterpenoids from Lycopus europaeus [J].
Hussein, AA ;
Rodríguez, B .
JOURNAL OF NATURAL PRODUCTS, 2000, 63 (03) :419-421