Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins

被引：19

作者：

Haynes, Lia M.

Miao, Congrong

Harcourt, Jennifer L.

Montgomery, Joel M.

Le, Mai Quynh

Dryga, Sergey A.

Kamrud, Kurt I.

Rivers, Bryan

Babcock, Gregory J.

Oliver, Jennifer Betts

Comer, James A.

Reynolds, Mary

Uyeki, Timothy M.

Bausch, Daniel

Ksiazek, Thomas

Thomas, William

Alterson, Harold

Smith, Jonathan

Ambrosino, Donna M.

Anderson, Larry J.

机构：

[1] Ctr Dis Control & Prevent, Natl Ctr Immunizat & Resp Dis, Div Viral Dis, Rep & Gastroenteritis Viruses Branch, Atlanta, GA 30333 USA

[2] Ctr Dis Control & Prevent, Special Pathogens Branch, Div Viral & Rickettsial Dis, Atlanta, GA 30333 USA

[3] Ctr Dis Control & Prevent, Influenza Div, Atlanta, GA 30333 USA

[4] AlphaVax Inc, Res Triangle Pk, NC USA

[5] Univ Massachusetts, Sch Med, Biol Labs, Jamaica Plain, MA USA

[6] Natl Inst Hyg & Epidemiol, Hanoi, Vietnam

来源：

CLINICAL AND VACCINE IMMUNOLOGY | 2007年 / 14卷 / 03期

关键词：

D O I：

10.1128/CVI.00351-06

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Recombinant severe acute respiratory syndrome (SARS) nucleocapsid and spike protein-based immunoglobulin G immunoassays were developed and evaluated. Our assays demonstrated high sensitivity and specificity to the SARS coronavirus in sera collected from patients as late as 2 years postonset of symptoms. These assays will be useful not only for routine SARS coronavirus diagnostics but also for epidemiological and antibody kinetic studies.

引用

页码：331 / 333

页数：3

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