Identification, expression and functional characterisation of CYP1A in grass carp (Ctenopharyngodon idella)

被引：3
作者：
Chu, Pengfei [1 ,2 ]
He, Libo [1 ]
Zhu, Denghui [1 ,2 ]
Huang, Rong [1 ]
Liao, Lanjie [1 ]
Li, Yongming [1 ]
Zhu, Zuoyan [1 ]
Wang, Yaping [1 ,3 ]
机构：
[1] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[3] Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China
来源：
FISH & SHELLFISH IMMUNOLOGY | 2019年 / 95卷
基金：
中国国家自然科学基金;
关键词：
CYP1A; Grass carp (Ctenopharyngodon idella); Immunity; Subcellular localisation; IFN-I; GENETIC POLYMORPHISMS; CYTOCHROME-P450; CYP; SIGNALING PATHWAYS; INDUCTION; ENZYMES; ADENOCARCINOMA; ASSOCIATION; CARCINOMA; OXIDATION; CHEMICALS;
D O I：
10.1016/j.fsi.2019.10.022
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
In mammal, CYP1A has attracted special attention due to its important roles in the oxidative metabolism. In fish, the researches on CYP1A are more focus on its roles in pollution in water environments, but the immune function is unclear. In the study, CiCYP1A gene was cloned from grass carp (Ctenopharyngodon idella). Tissue distribution exhibited an overwhelmingly high basal expression levels in the liver. After GCRV infection, CiCYP1A showed a potent response, indicating CiCYP1A was involved in GCRV-induced immunity. Subcellular localisation showed CiCYP1A was distributed in the cytoplasm. Besides, dual-luciferase activity assays revealed CYP1A was relevant for IFN-I signaling pathway modulation, furthermore, overexpressed CYP1A potently suppressed the mRNA expression of IRF3 and IFN-I but not IRF7. The results provide new sights into exploring immune function of CiCYP1A in teleosts.
引用
收藏
页码：35 / 43
页数：9
相关论文
共 48 条
  • [1] Cytochrome P450 CYP1A1: wider roles in cancer progression and prevention
    Androutsopoulos, Vasilis P.
    Tsatsakis, Aristidis M.
    Spandidos, Demetrios A.
    [J]. BMC CANCER, 2009, 9
  • [2] CYP1A2 genetic polymorphisms and adenocarcinoma lung cancer risk in the Tunisian population
    B'chir, Fatma
    Pavanello, Sofia
    Knani, Jalel
    Boughattas, Sami
    Arnaud, Maurice J.
    Saguem, Saad
    [J]. LIFE SCIENCES, 2009, 84 (21-22) : 779 - 784
  • [3] Banerjee B D, 2001, Rev Environ Health, V16, P1
  • [4] CYP1A inhibition in fish gill filaments: A novel assay applied on pharmaceuticals and other chemicals
    Beijer, Kristina
    Abrahamson, Alexandra
    Brunstrom, Bjorn
    Brandt, Ingvar
    [J]. AQUATIC TOXICOLOGY, 2010, 96 (02) : 145 - 150
  • [5] Chen XP, 2006, PHARMACOGENET GENOM, V16, P219
  • [6] China's Ministry of Agriculture, 2018, CHIN FISH STAT YB
  • [7] Characterisation and function of TRIM23 in grass carp (Ctenopharyngodon idella)
    Chu, Pengfei
    He, Libo
    Yang, Cheng
    Li, Yangyu
    Huang, Rong
    Liao, Lanjie
    Li, Yongming
    Zhu, Zuoyan
    Wang, Yaping
    [J]. FISH & SHELLFISH IMMUNOLOGY, 2019, 88 : 627 - 635
  • [8] Molecular cloning and functional characterisation of NLRX1 in grass carp (Ctenopharyngodon idella)
    Chu, Pengfei
    He, Libo
    Li, Yangyang
    Huang, Rong
    Liao, Lanjie
    Li, Yongming
    Zhu, Zuoyan
    Wang, Yaping
    [J]. FISH & SHELLFISH IMMUNOLOGY, 2018, 81 : 276 - 283
  • [9] Oncogenic signaling pathways activated in DMBA-induced mouse mammary tumors
    Currier, N
    Solomon, SE
    Demicco, EG
    Chang, DLF
    Farago, M
    Ying, HQ
    Dominguez, I
    Sonenshein, GE
    Cardiff, RD
    Xiao, ZXJ
    Sherr, DH
    Seldin, DC
    [J]. TOXICOLOGIC PATHOLOGY, 2005, 33 (06) : 726 - 737
  • [10] EROD activity and antioxidant defenses of sea bass (Dicentrarchus labrax) after an in vivo chronic hydrocarbon pollution followed by a post-exposure period
    Danion, Morgane
    Le Floch, Stephane
    Lamour, Francois
    Quentel, Claire
    [J]. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2014, 21 (24) : 13769 - 13778
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