Enhancement of anti-tumor activity of gamma-irradiated silk fibroin via immunomodulatory effects

被引：38

作者：

Byun, Eui-Baek ^{[1
,2
]}

Sung, Nak-Yun ^{[1
]}

Kim, Jae-Hun ^{[1
]}

Choi, Jong-il ^{[1
]}

Matsui, Toshiro ^{[2
]}

Byun, Myung-Woo ^{[3
]}

Lee, Ju-Woon ^{[1
]}

机构：

[1] Korea Atom Energy Res Inst, Adv Radiat Technol Inst, Jeongeup 580185, South Korea

[2] Kyushu Univ, Div Bioresources & Biosci, Fac Agr, Grad Sch, Fukuoka 8128581, Japan

[3] Woosong Univ, Dept Food Sci & Biotechnol, Taejon 300718, South Korea

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 2010年 / 186卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

Fibroin; Gamma irradiation; Immune response; Cytotoxicity; NK cell; Cytokine; NECROSIS-FACTOR-ALPHA; IN-VITRO; TUMOR; DOXORUBICIN; INHIBITION; MECHANISM; CELLS;

D O I：

10.1016/j.cbi.2010.03.032

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Silk fibers have proven to be effective in many clinical applications as well as for clothing. In addition to the substantial effect of silk fibers, the present study was conducted to explore its importance in a new dimension to reinforce the effects of its physiological function regarding anti-tumor activity and immune response with gamma-irradiated silk fibroin (GISF). The cytotoxicity results showed that pre-treatment of GISF in the mouse peritoneal macrophages (MPM) indicated a higher proliferative effect than that of non-irradiated silk fibroin (NISF) in a concentration-dependent manner. Based on the cytotoxicity result of MPM, GISF (50 and 150 kGy) was selected for an ex vivo study in an animal (C57BL6) system and evaluated about whether the non-specific immune response was also related to GISF. GISF (50 and 150 kGy) augmented immune responsiveness via activation of NK cells, T lymphocytes proliferation. NO production. and cytokine level, such as IL-6, IL-2, IL-12, IFN-gamma, TNF-alpha, as compared with NISF, which strongly suggested that GISF significantly augmented an important element of all aspects of the innate and adaptive immune system. Therefore, from these results, it seems likely that the GISF will play a potent role in eliciting the effect of the non-specific immune response and anti-tumor activity as a value-added product in the medical industry. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

引用

页码：90 / 95

页数：6

共 28 条

[1]

BARLOZZARI T, 1985, J IMMUNOL, V134, P2783

[2]

Baxevanis CN, 2000, EUR J IMMUNOL, V30, P1957, DOI 10.1002/1521-4141(200007)30:7<1957::AID-IMMU1957>3.0.CO

[3]

2-7

[4] In vitro and in vivo studies on the cytotoxicity of irradiated silk fibroin against mouse melanoma tumor cell [J].

Byun, Eui-Baek ;

Sung, Nak-Yun ;

Kwon, Sun-Kyu ;

Song, Beom-Seok ;

Kim, Jae-Hun ;

Choi, Jong-il ;

Hwang, Han-Joon ;

Byun, Myung-Woo ;

Lee, Ju-Woon .

RADIATION PHYSICS AND CHEMISTRY, 2009, 78 (7-8) :429-431

[5]

Byun MW, 1994, RADIOISOTOPE NEWS, V9, P32

[6] Cytokine production, lymphocyte proliferation and T-Cell receptor Vβ expression in primary peripheral blood mononuclear cell cultures from nickel-allergic individuals [J].

Cederbrant, K ;

Anderson, C ;

Andersson, T ;

Marcusson-Ståhl, M ;

Hultman, P .

INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 2003, 132 (04) :373-379

[7] Drug release rate influences the pharmacokinetics, biodistribution, therapeutic activity, and toxicity of pegylated liposomal doxorubicin formulations in murine breast cancer [J].

Charrois, GJR ;

Allen, TM .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2004, 1663 (1-2) :167-177

[8] Silk fibroin/poly(carbonate)-urethane as a substrate for cell growth: in vitro interactions with human cells [J].

Chiarini, A ;

Petrini, P ;

Bozzini, S ;

Dal Pra, I ;

Armato, U .

BIOMATERIALS, 2003, 24 (05) :789-799

[9]

Field CJ, 2000, AM J CLIN NUTR, V71, p1720S, DOI 10.1093/ajcn/71.6.1720S

[10] The role of NK cell recognition of nectin and nectin-like proteins in tumor immunosurveillance [J].

Fuchs, Anja ;

Colonna, Marco .

SEMINARS IN CANCER BIOLOGY, 2006, 16 (05) :359-366

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