Laserspray Ionization (LSI) Ion Mobility Spectrometry (IMS) Mass Spectrometry

被引：47

作者：

Inutan, Ellen ^{[1
]}

Trimpin, Sarah ^{[1
]}

机构：

[1] Wayne State Univ, Dept Chem, Detroit, MI 48202 USA

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2010年 / 21卷 / 07期

基金：

美国国家科学基金会;

关键词：

UBIQUITIN IONS; MS; RESOLUTION; PEPTIDES; PROTEINS; STATES; TOF;

D O I：

10.1016/j.jasms.2010.03.039

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple device is described for desolvation of highly charged matrix/analyte clusters produced by laser ablation leading to multiply charged ions that are analyzed by ion mobility spectrometry-mass spectrometry. Thus, for example, highly charged ions of ubiquitin and lysozyme are cleanly separated in the gas phase according to size and mass (shape and molecular weight) as well as charge using Tri-Wave ion mobility technology coupled to mass spectrometry. This contribution confirms the mechanistic argument that desolvation is necessary to produce multiply charged matrix-assisted laser desorption/ionization (MALDI) ions and points to how these ions can be routinely formed on any atmospheric pressure mass spectrometer. (J Am Soc Mass Spectrom 2010, 21, 1260-1264) (C) 2010 American Society for Mass Spectrometry

引用

收藏

页码：1260 / 1264

页数：5

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