The mouse inhibin alpha-subunit promoter directs SV40 T-antigen to Leydig cells in transgenic mice

被引：62

作者：

Kananen, K ^{[1
]}

Markkula, M ^{[1
]}

ElHefnawy, T ^{[1
]}

Zhang, FP ^{[1
]}

Paukku, T ^{[1
]}

Su, JGJ ^{[1
]}

Hsueh, AJW ^{[1
]}

Huhtaniemi, I ^{[1
]}

机构：

[1] STANFORD UNIV,SCH MED,DEPT GYNECOL & OBSTET,DIV REPROD BIOL,STANFORD,CA 94305

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1996年 / 119卷 / 02期

关键词：

inhibin alpha; Leydig cell; LH action; SV40; T-antigen; testis;

D O I：

10.1016/0303-7207(96)03802-6

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Testicular tumorigenesis was observed in transgenic mice expressing the 6-kb mouse inhibin cr-subunit promoter/Simian virus 40 T-antigen (SV40 Tag) fusion gene. The tumors were confined to Leydig cells using immunohistochemistry with anti-Tag antibody, specific binding of biotinylated hCG and histochemistry for 3 beta-hydroxysteroid dehydrogenase. Leydig cell hyperplasia and presence of Tag protein in the testicular interstitial tissue were already evident at 5 and 6.5 days of age, respectively. An immortalized cell line, BLT-1, was established from one testicular tumor. These cells expressed the LH receptor and P450soc mRNAs, and displayed LH-responsive cAMP and progesterone production, and low testosterone production. The cells also specifically bound I-125-labeled recombinant human LK with high affinity (36 000 binding sites/cell), and the binding was regulated by 8Br-cAMP and hCG. This gonadal tumor model is valuable for further studies on endocrine functions of Leydig cells and their tumorigenesis in vivo and in vitro.

引用

页码：135 / 146

页数：12

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