Live imaging of X chromosome inactivation and reactivation dynamics

被引：2
作者：
Kobayashi, Shin [1 ,2 ]
机构：
[1] Natl Inst Adv Ind Sci & Technol, Mol Profiling Res Ctr Drug Discovery, Koutou Ku, 2-4-7 Aomi, Tokyo 1350064, Japan
[2] Tokyo Med & Dent Univ, Med Res Inst, Dept Epigenet, Bunkyo Ku, 1-5-45 Yushima, Tokyo 1138510, Japan
来源：
DEVELOPMENT GROWTH & DIFFERENTIATION | 2017年 / 59卷 / 06期
基金：
日本科学技术振兴机构;
关键词：
epigenetics; mammalian development; pluripotent stem cells; reprogramming; X chromosome inactivation; EARLY MOUSE EMBRYOS; PLURIPOTENT STEM-CELLS; PRIMORDIAL GERM-CELLS; DOSAGE COMPENSATION; FEMALE MOUSE; EARLY POSTIMPLANTATION; LINKED LACZ; IN-VITRO; GENE; EXPRESSION;
D O I：
10.1111/dgd.12365
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The epigenetic phenomenon called X chromosome inactivation plays critical roles in female development in eutherian mammals, and has attracted attention in the fields of developmental biology and regenerative biology in efforts to understand the pluripotency of stem cells. X chromosome inactivation is routinely studied after cell fixation, but live imaging is increasingly being required to improve our understanding of the dynamics and kinetics of X chromosome inactivation and reactivation processes. Here, we describe our live imaging method to monitor the epigenetic status of X chromosomes using a gene knock-in mouse strain named "Momiji" and give an overview of the application of this strain as a resource for biological and stem cell research.
引用
收藏
页码：493 / 500
页数：8
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