Gene-specific trans-regulatory functions of magnesium for chloroplast mRNA stability in higher plants

被引：29

作者：

Horlitz, M ^{[1
]}

Klaff, P ^{[1
]}

机构：

[1] Univ Dusseldorf, Inst Phys Biol, D-40225 Dusseldorf, Germany

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2000年 / 275卷 / 45期

关键词：

D O I：

10.1074/jbc.M005622200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In higher plant chloroplasts the accumulation of plastid-encoded mRNAs during leaf maturation is regulated via gene-specific mRNA stabilization. The half-lives of chloroplast RNAs are specifically affected by magnesium ions. psbA mRNA (D1 protein of photosystem II), rbcL mRNA (large subunit of ribulose-1,5-bisphosphate carboxylase), 16 S rRNA, and tRNA(His) gain stability at specific magnesium concentrations in an in vitro degradation system from spinach chloroplasts. Each RNA exhibits a typical magnesium concentration-dependent stabilization profile. It shows a cooperative response of the stability-regulated psbA mRNA and a saturation curve for the other RNAs. The concentration of free Mg2+ rises during chloroplast development within a range sufficient to mediate gene-specific mRNA stabilization in vivo as observed in vitro. We suggest that magnesium ions are a trans-acting factor mediating differential mRNA stability.

引用

页码：35638 / 35645

页数：8

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