Circular RNA hsa_circ_0001649 inhibits hepatocellular carcinoma progression via multiple miRNAs sponge

被引:66
作者
Su, Yang [1 ,2 ]
Xu, Chao [1 ]
Liu, Yuting [1 ]
Hu, Yilin [3 ]
Wu, Haiyan [1 ]
机构
[1] Nanjing Med Univ, Dept Hepatobiliary & Pancreat Surg, Affiliated Huaian Peoples Hosp 1, Huaian, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Ctr Global Hlth, Key Lab Modern Toxicol,Minist Educ,Sch Publ Hlth, State Key Lab Reprod Med, Nanjing, Jiangsu, Peoples R China
[3] Nantong Univ, Affiliated Hosp, Res Ctr Clin Med, Nantong, Jiangsu, Peoples R China
来源
AGING-US | 2019年 / 11卷 / 10期
关键词
circRNA; proliferation; migration; hepatocellular carcinoma; ceRNA; CELL-GROWTH; EXPRESSION; GENE;
D O I
10.18632/aging.101988
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Circular RNA (circRNA) exerts an essential role in tumor development. Hsa_circ_0001649 (circ-0001649) was produced at the SHPRH gene locus containing exon 26-29. This study analyzed the specific mechanism of circ-0001649 in influencing the development of hepatocellular carcinoma (HCC). Relative levels of circ-0001649 in HCC cell lines and tissues were examined by qRT-PCR. The direct binding between circ-0001649 and miR-127-5p/miR-612/miR-4688 were verified through Dual-luciferase reporter gene assay, RNA Binding Protein Immunoprecipitation (RIP) assay and western blot detection. In vitro and in vivo regulatory roles of circ-0001649 in proliferative and migratory abilities of HCC were evaluated by EdU, Transwell and tumourigenicity assay, respectively. Results showed that circ-0001649 was markedly decreased in hepatocellular carcinoma cell lines and tumor tissues. Overexpression of circ-0001649 greatly inhibited proliferation and migration of HCC in vitro and in vivo. More importantly, we confirmed that circ-0001649 regulated cellular behaviors of HCC cells by targeting SHPRH. Furthermore, we determined that circ-0001649 served as a ceRNA to sponge miR-127-5p, miR-612 and miR-4688, thus activating SHPRH. In summary, our study showed that circ-0001649 was lowly expressed in HCC and inhibited HCC progression via multiple miRNAs sponge.
引用
收藏
页码:3362 / 3375
页数:14
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