Differential epigenetic regulation of BDNF and NT-3 genes by trichostatin A and 5-aza-2′-deoxycytidine in Neuro-2a cells

被引：43
作者：
Ishimaru, Naoki [1 ,2 ]
Fukuchi, Mamoru [2 ]
Hirai, Akina [1 ]
Chiba, Yusuke [2 ]
Tamura, Tomonari [1 ]
Takahashi, Nami [1 ]
Tabuchi, Akiko [2 ]
Tsuda, Masaaki [2 ]
Shiraishi, Masahiko [1 ]
机构：
[1] Int Univ Hlth & Welf, Fac Pharmaceut Sci, Otawara, Tochigi 3248501, Japan
[2] Toyama Univ, Grad Sch Med & Pharmaceut Sci, Fac Pharmaceut Sci, Dept Biol Chem, Toyama 9300194, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 394卷 / 01期
关键词：
DNA methylation; Histone acetylation; BDNF; NT-3; NEUROTROPHIC FACTOR; CALCIUM REGULATION; CORTICAL-NEURONS; DNA METHYLATION; BINDING PROTEIN; NERVOUS-SYSTEM; EXPRESSION; TRANSCRIPTION; BRAIN; MOUSE;
D O I：
10.1016/j.bbrc.2010.02.139
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To understand epigenetic regulation of neurotrophins in Neuro-2a mouse neuroblastoma cells, we investigated the alteration of CpG methylation of brain-derived neurotrophic factor (BDNF) promoter I and neurotrophin-3 (NT-3) promoter IB and that of histone modification in Neuro-2a cells. Bisulfite genomic sequencing showed that the CpG sites of BDNF promoter I were methylated in non-treated Neuro-2a cells and demethylated following 5-aza-2'-deoxycytidine (5-aza-dC) treatment. In contrast, methylation status of the NT-3 promoter IB did not change by 5-aza-dC treatment in Neuro-2a cells. Furthermore, we demonstrated that BDNF exon I-IX mRNA was induced by trichostatin A (TSA) treatment. However, NT-3 exon IB-II mRNA was not induced by TSA treatment. Chromatin immunoprecipitation assays showed that the levels of acetylated histones H3 and H4 on BDNF promoter I were increased by TSA. These results demonstrate that DNA methylation and/or histone modification regulate BDNF gene expression, but do not regulate NT-3 gene expression in Neuro-2a cells. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码：173 / 177
页数：5
相关论文
共 28 条
  • [1] Mouse and rat BDNF gene structure and expression revisited
    Aid, Tamara
    Kazantseva, Anna
    Piirsoo, Marko
    Palm, Kaia
    Timmusk, Tonis
    [J]. JOURNAL OF NEUROSCIENCE RESEARCH, 2007, 85 (03) : 525 - 535
  • [2] PURIFICATION OF A NEW NEUROTROPHIC FACTOR FROM MAMMALIAN BRAIN
    BARDE, YA
    EDGAR, D
    THOENEN, H
    [J]. EMBO JOURNAL, 1982, 1 (05) : 549 - 553
  • [3] Brain-derived neurotrophic factor
    Binder, DK
    Scharfman, HE
    [J]. GROWTH FACTORS, 2004, 22 (03) : 123 - 131
  • [4] Normal huntingtin function: An alternative approach to Huntington's disease
    Cattaneo, E
    Zuccato, C
    Tartari, M
    [J]. NATURE REVIEWS NEUROSCIENCE, 2005, 6 (12) : 919 - 930
  • [5] Neurotrophin signalling in health and disease
    Chao, MV
    Rajagopal, R
    Lee, FS
    [J]. CLINICAL SCIENCE, 2006, 110 (02) : 167 - 173
  • [6] Derepression of BDNF transcription involves calcium-dependent phosphorylation of MeCP2
    Chen, WG
    Chang, Q
    Lin, YX
    Meissner, A
    West, AE
    Griffith, EC
    Jaenisch, R
    Greenberg, ME
    [J]. SCIENCE, 2003, 302 (5646) : 885 - 889
  • [7] Chen WG, 2003, J NEUROSCI, V23, P2572
  • [8] Epigenetic mechanisms and gene networks in the nervous system
    Colvis, CM
    Pollock, JD
    Goodman, RH
    Impey, S
    Dunn, J
    Mandel, G
    Champagne, FA
    Mayford, M
    Korzus, E
    Kumar, A
    Renthal, W
    Theobald, DEH
    Nestler, EJ
    [J]. JOURNAL OF NEUROSCIENCE, 2005, 25 (45) : 10379 - 10389
  • [9] Regional expression of brain derived neurotrophic factor (BDNF) is correlated with dynamic patterns of promoter methylation in the developing mouse forebrain
    Dennis, KE
    Levitt, P
    [J]. MOLECULAR BRAIN RESEARCH, 2005, 140 (1-2): : 1 - 9
  • [10] Neurotrophic factors as a therapeutic target for Parkinson's disease
    Evans, Jonathan R.
    Barker, Roger A.
    [J]. EXPERT OPINION ON THERAPEUTIC TARGETS, 2008, 12 (04) : 437 - 447
123