Detection of Ara h 1 (a major peanut allergen) in food using an electrochemical gold nanoparticle-coated screen-printed immunosensor

被引：71

作者：

Alves, Rita C. ^{[1
,2
]}

Pimentel, Filipa B. ^{[2
]}

Nouws, Henri P. A. ^{[1
]}

Marques, Raquel C. B. ^{[1
]}

Gonzalez-Garcia, Maria Begona ^{[1
]}

Oliveira, M. Beatriz P. P. ^{[2
]}

Delerue-Matos, Cristina ^{[1
]}

机构：

[1] Inst Politecn Porto, Inst Super Engn Porto, REQUIMTE, P-4200072 Oporto, Portugal

[2] Univ Porto, Fac Farm, Dept Ciencias Quim, REQUIMTE, P-4050313 Oporto, Portugal

来源：

BIOSENSORS & BIOELECTRONICS | 2015年 / 64卷

关键词：

Electrochemical immunosensor; Biosensor; Screen-printed carbon electrode; Allergen; Peanut; Ara h 1; PROTEIN; BIOSENSOR;

D O I：

10.1016/j.bios.2014.08.026

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A gold nanoparticle-coated screen-printed carbon electrode was used as the transducer in the development of an electrochemical immunosensor for Ara h 1 (a major peanut allergen) detection in food samples. Gold nanoparticles (average diameter=32 nm) were electrochemically generated on the surface of screen-printed carbon electrodes. Two monoclonal antibodies were used in a sandwich-type immunoassay and the antibody-antigen interaction was electrochemically detected through stripping analysis of enzymatically (using alkaline phosphatase) deposited silver. The total time of the optimized immunoassay was 3 h 50 min. The developed immunosensor allowed the quantification of Ara h 1 between 12.6 and 2000 ng/ml, with a limit of detection of 3.8 ng/ml, and provided precise (RSD <8.7%) and accurate (recovery > 96.6%) results. The immunosensor was successfully applied to the analysis of complex food matrices (cookies and chocolate), being able to detect Ara h 1 in samples containing 0.1% of peanut. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：19 / 24

页数：6

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